Performance of Caffeine Content Analysis in Robusta sp and Theobroma cacao L using Iodometry, UV-Vis Spectrophotometry and High Performance Liquid Chromatography
Yeanchon H. Dulanlebit, Nikmans Hattu, Ritti P. Sangadji, Risma Mahulette, Nataniel Kelmanutu, Sandriliati Asari
J. Pure App. Chem. Res. Vol 9, No 3 (2020), pp. 212-221
Submitted: May 04, 2020     Accepted: December 20, 2020     Published: December 25, 2020
DOI: http://dx.doi.org/10.21776/ub.jpacr.2020.009.03.535

Abstract


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Caffeine is a xanthine alkaloid compound found in Robusta sp and Theobroma cacao L. The caffeine extract was obtained by evaporating CHCl3 using a rotary evaporator and determine its content using iodometry, UV-Vis spectrophotometry and High Performance Liquid Chromatography, then validated by measurement methods. The iodometric caffeine content in Theobroma cacao L is 900 mg/kg while UV-Vis spectrophotometry is 4,000 mg/kg, and for HPLC in Robusta sp is 19,475 mg/kg. The iodometry performance gives the linearity value, R2 of 0.9791. The precision values at 5.0 mg/L and 15.0 mg/L are 4.6% and 8.3%. Accuracy for 5.0; 10.0; and 15.0 mg/L are 106%, 100% and 94% respectively. The  performance of UV-Vis spectrophotometry gives  linearity value, R2 of 0.9948. The precision value at 1.0 mg/L and 6.0 mg/L gives the variance coefficient value of 4.2% and 1.2%, while the accuracy at 1.0 mg/L and 6.0 mg/L are 99% and 101%.



Keywords : caffeine, HPLC, iodometry, Robusta sp, spectrophotometry, Theobroma cacao L
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