Phytochemistry and Antidiabetic Evaluation in-vitro and in-vivo of Ethyl Acetate Fractions from Sungkai Leaves (Peronema canescens Jack)
Qurrota Aini, Madyawati Latief, Indra Lasmana Tarigan, Masruri MASRURI
J. Pure App. Chem. Res. Vol 13, No 2 (2024), pp. 127-136
Submitted: July 23, 2022     Accepted: August 29, 2024     Published: August 29, 2024

Abstract


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The paper reports the in vivo and in vitro antidiabetic activity of bioactive compounds composed of ethyl acetate fractions from Sungkai (Peronema canescens Jack). The fractions are obtained from vacuum liquid chromatography (F1, F2 and F3). Phytochemicals screening, UV-Vis and FTIR spectrometry analysis is applied to detect secondary metabolite composed of the fractions. In addition, in vitro antidiabetic capability is measured inhibiting a-amylase activity, and in vivo evaluation is conducted using diabetic rat's model induced by alloxan. It was found that fraction collected as a white precipitate. The phytochemical analysis indicated positive for flavonoid. UV-Vis and FTIR spectra support the data. Moreover, capability of the fractions to inhibit a-glucosidase activity in the range 5-100 ppm does not show the activity. Conversely, the in vivo evaluation from fraction 2 at concentration 600 mg/Kg of body weight has antidiabetic in 50.16%. This result comparable to that of glibenclamide as a control. This finding lead to further evaluation for structural elucidation.


Keywords : Antidiabetic; sungkai leaves; Ethyl acetate fractions;
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References


Krisnatuti, D., D. Rasjmida dan R. Yenrina, Diet Sehat untuk Penderita Diabetes Melitus, 2014, Penebar Swadaya, Jakarta. [2] International Diabetes Federation, IDF Diabetes Atlas, 9th ed., 2019, International Diabetes Federation, Brussels, Belgium. [3] Latief, M., Fisesa., A. T., Sari., P. M. and Tarigan, I. L., Jurnal Farmasi Sains dan Praktis, 2021, 7(2), 144–153. [4] Ningsih, A. and Ibrahim, A., J. Trop. Phar. Chem., 2013. 2(2), 76–82. [5] Andriani, F., Sundaryono, A. and Nurhamidah, N., Jurnal Pendidikan dan Ilmu Kimia, 2017, 1(1), 33–38. [6] Oktiansyah, R., Elfita, E., Widjajanti, H., Salni, S. and Setiawan, A., Biodivers. J., 2023, 24(12), 6516-6526. [7] Latief, M., Tarigan, I. L., Sari, P. M., and Aurora, F. E., Jurnal Farmasi Indonesia 2021, 18(1), 23–37. [8] Latief, M., Sari, P. M., Fatwa, L. T., Tarigan, I. L. and Rupasinghe, H.P.V., Pharmacol. Clin. Pharm. Res., 2021, 6(2), 64-74. [9] Ramadenti, F., Sundaryono, A. and Handayani, D., Alotrop Jurnal Pendidikan Ilmu Kimia, 2017, 1(2), 89–92. [10] Suryani, A.F., Nisa, K., Indrianingsih, A.W., Handayani, S., Fitrotin, U., Rahayu, E., Wulandari, A.A. and Wijayanti, N., IOP Conf. Ser.: Earth. Environ. Sci., 2024, 1377, 1, 012060. [11] Pratiwi, U., Muharni, M., Ferlinahayati, F. and Yohandini, H., AIP Conf. Proceed., 2023, 2913, 1, 060007. [12] Harborne, J. B, Metode Fitokimia: Penuntun Cara Modern Menganalisis Tumbuhan, 1982, ITB, Bandung. [13] Mistry, J., Biswas, M., Sarkar, S. and Ghosh, S., Future J. Pharm. Sci., 2023, 9(1), 22. [14] Masruri, M., Pangestin, D.N., Pangesti, P.A. and Arini, W., IOP Conf. Ser.: Earth Environ. Sci., 2019, 239 (1), 012025. [15] Cam, M., Basyigit, B., Alasalyar, H., Yilmaztekin, M., Ahhmed, A., Sagdic, O., Konca, Y. and Telci, I., Food Biosci., 2020, 35, 100577. [16] Wang, M., Wang, W., Zhang, X., Dai, G. and Tang, K., Talanta, 2024, 275, 126121. [17] Palmieri, S., Pellegrini, M. Ricci, A., Compagnone, D. and Lo Sterzo, C., Food, 2020, 9 (9), 1221. [18] Mondal, M., Saha, S., Hossain, M., Al Foyjul, I., Sarkar, C., Hossain, S., Khalipha, A.B.R. and Kundu, S.K., J. Herbs Spices Med. Plants, 2020, 26(4), 405-422. [19] Ku Halim, K. F., Jalani, K.J., Mohsin, H.F. and Abdul Wahab, I., International Journal of Pharmaceutical, Nutraceutical and Cosmetic Science (IJPNaCS), 2020, 1, 7-15. [20] Summayya, P.C., Jalala, V.K., Ahammed, T.S. and Muraleedharan, K., Comput. Biol. Chem., 2022, 101, 107777. [21] Lenzen, S., Diabetologia, 2008, 51(2), 216-226. [22] Kung, T.F., Wilkinson, C.M., Liddle, L.J. and Colbourne, F., Plos one, 2023, 18(9), e0292033. [23] Tarigan, I.L., Triani, A.A. and Latief, M., J. Pure App. Chem. Res., 2023, 12(2) 117-127. [24] Syofyan, S., Wulandari, A., Alen, Y., Diliarosta, S., Kurniawan, H., Noverial, N., Putra, P.P. and Dillasamola, D., T. J. Nat. Prod. Res., 2023, 7(5), 2863-2866. [25] Bondonno, N.P., Dalgaard, F., Murray, K. Davey, R.J., Bondonno, C.P., Cassidy, A., Lewis, J.R., Kyro, C., Gislason, G., Scalbert, A. and Tjonneland, A., J. Nutr., 2021, 151(11), 3533-3542. [26] Rahmi, A. and Arcot, J., Foods, 2023, 12(3), 631. [27] Miller, N. and Joubert, E., Planta Med., 2022, 88(12), 1078-1091.


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