Antidiabetic Activity of the Methanol Fraction of Sungkai Leaves (Peronema canescens Jack)
Indra Lasmana Tarigan, Anggi Ayunda Triani, Madyawati Latief
J. Pure App. Chem. Res. Vol 12, No 2 (2023), pp.
Submitted: July 20, 2022     Accepted: August 30, 2023     Published: August 30, 2023

Abstract



Diabetes Mellitus (DM) is one of a group of metabolic diseases characterized by hyperglycemia caused by a disturbance in insulin so that blood glucose levels increase. Prevention of absorption of blood glucose by the intestine can be done with the help of the enzyme α-glucosidase. Based on the results of phytochemical tests, it is known that the methanol fraction of sungkai leaves contains flavonoid compounds. The Fourier Transform Infra-Red (FTIR) spectrum showed that the isolates had phenolic O-H groups (3,354.53 cm-1, 1,359.28 cm-1), aromatic C=C group (1,615.48 cm-1), C-O-C ether group (1,046.60 cm-1) and aromatic C-H group (822.21 cm-1). In vivo antidiabetic activity test was carried out using test animals of white male mice which were induced by alloxan. Antidiabetic testing was carried out using 6 treatment groups with glibenclamide, Na-Carboxymethyl cellulose (Na-CMC) 0.5%, dose 175 mg/kgBW, 350 mg/kgBW, 700 mg/kg Body Weight (BW) and isolates 2 mg/kgBW. The results showed that the methanol fraction of sungkai leaves had the best antidiabetic activity at a dose of isolate and 700 mg/kgBW which was able to reduce blood glucose levels by 42.20% and 42.00%. In vitro antidiabetic testing through α-glucosidase enzyme inhibition mechanism did not show any antidiabetic activity at concentrations of fractions 5, 10, 25, 50 and 100 ppm

Keywords : Diabetes Melitus; Antidiabetic; Sungkai Leaves; Flavonoid


References


[1] U. Galicia-Garcia, A. Benito-Vicente, S. Jebari, et al. Int. J. Mol. Sci., vol. 21 (17), 1–34, 2020.

[2] M. E. Craig, A. Hattersley, & K. C. Donaghue, Pediatr. Diabetes, vol. 10 (12), 3–12, 2009.

[3] H. Arifin, K. R. Chou, K. Ibrahim, et al., J. Multidiscip. Healthc., vol. 15, 2203–2221, 2022.

[4] M. Z. Banday, A. S. Sameer, & S. Nissar, Avicenna J. Med., vol. 10 (4), 174–188, 2020.

[5] S. Sinulingga, Subandrate, & Safyudin, J. Kedokt. & Kesehat., vol. 16 (1), 76–83, 2020.

[6] S. G. Sari, R. Rahmawati, Rusmiati, & Susi, EnviroScienteae, vol. 19 (1), 1–23, 2023.

[7] R. H. Kusriani, A. Nawawi, & T. Turahman, J. Farm. Galen., vol. 2 (1), 8–14, 2015.

[8] A. Fenny, A. Sundaryono, & Nurhamidah, Alotrop J. Pendidik. & Ilmu Kim., vol. 1(1), 33–38, 2017.

[9] M. Latief, Lizawati, I. L. Tarigan, et al., AIP Conf. Proc., vol. 2595 (080004), 2023.

[10] I. Ahmad & A. Ibrahim, J. Sains & Kesehat., vol. 1 (3), 114–119, 2015.

[11] D. Prasiwi, A. Sundaryono, & D. Handayani, Alotrop J. Pendidik. & Ilmu Kim., vol. 2, (2), 25–32, 2018.

[12] M. Fikriansyah, M. Latief, and I. L. Tarigan, Chempublish J., vol. 7 (1), 54–63, 2023.

[13] N. A. P. Pakpahan, “Isolasi dan Uji Aktivitas Antikolesterol Senyawa Metabolit Sekunder dari Fraksi n-Heksana Daun Sungkai (Peronema canescens Jack),” Bachelor Thesis, Sriwijaya University, Palembang, 2021.

[14] M. Latief, P. M. Sari, L. T. Fatwa, et al., Pharmacol. Clinic. Pharm. Res., vol. 6 (2), 64, 2021.

[15] N. Sitepu, Asian J. Pharm. Res. Dev., vol. 8 (3), 57–60, 2020.

[16] Z. A. Amani & R. Mustarichie, Farmaka, vol. 16 (1), 127–132, 2018.

[17] I. L. Tarigan, S. Sutrisno, R. Rumaida, et al., Pharmacogn. J., vol. 14 (6), 744–752, 2022.

[18] B. Muthmainnah, Media Farm., vol. 8 (2), 23–28, 2017.

[19] J. Chen, S. Mangelinckx, A. Adams, et al., Nat. Prod. Commun., vol. 10 (1), 187–200, 2015.

[20] M. K. Ladan, J. Straus, E. Tavčar Benković, & S. Kreft, Sci. Rep., vol. 7 (1), 1–8, 2017.

[21] Mahmiah, Indo. J. Chem., vol. 6 (3), 312–315, 2006.

[22] R. K. Al-Ishaq, M. Abotaleb, P. Kubatka et al., Biomolecules, vol. 9 (9), 430, 2019.

[23] M. Megawati, S. Fajriah, E. Supriadi, & Widiyarti, J. Kefarmasian Indones., vol. 11 (1), 1–7, 2021.

[24] F. M. S. Putri, J. Kesehat. Madani Med., vol. 9 (2), 51–61, 2018.

[25] A. Rauf, S. Ningsi, & F. Suhaidarwati, J. Farmasi FIK UINAM, vol. 6(1), 55–65, 2018.

[26] J. M. D. Soares, A. E. B. P. Leal, J. C. Silva, et al., Pharmacogn. Mag., vol. 13 (62), 179–188, 2017.

[27] P. Garcia-Molina, F. Garcia-Molina, J. A. Teruel-Puche, et al., Molecules, vol. 27 (10), 2022.

[28] U. Hossain, A. K. Das, S. Ghosh, & P. C. Sil, Food Chem. Toxicol., vol. 145, 111738, 2020.

[29] P. R. Rehani, H. Iftikhar, M. Nakajima, et al., J. Diabetes Res., vol. 2019, 4267357, 2019.


Refbacks

  • There are currently no refbacks.


Creative Commons License
This work is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License.