The Effect of Klika Ongkea Extract (Mezzetia parviflora Becc.) on Pancreatic β-Cells Regeneration by Streptozotocin-Induced in Wistar Rats
Jangga - Jangga, Mansur - Mansur, Saparuddin - Latu
J. Pure App. Chem. Res. Vol 11, No 1 (2022), pp. 31 - 37
Submitted: September 14, 2021     Accepted: March 30, 2022     Published: March 30, 2022
DOI: http://dx.doi.org/10.21776/ub.jpacr.2022.011.01.618

Abstract


Cover Image

This study aimed to determine the effect of Klika ongkea (Mezzetia parviflora Becc.) extract on the regeneration of pancreatic β-cells on Wistar rats that were induced using streptozotocin. Streptozotocin (STZ) with a 40 mg/kg BW dose was used in this study. Eighty adult male Wistar rats were used for this study. The animals were divided into four groups, Group I, as a healthy controls; Group II as an STZ-induced, Group III, STZ-induced and treated with galvus (Vildagliptin) 0.9 mg/200 g BW; Group IV, STZ-induced and treated with Klika ongkea 100 mg/kg BW. This study was conducted for 28 days. Four animals each group on the 1st, 7th, 14th, 21st and 28th days were observed to analyze the number of pancreatic β-cells. On the last day, pancreases were isolated and stained with hematoxylin & eosin (H&E) to analyze the regeneration of pancreatic β-cells. The data analysis was performed using an Independent-Sample T-Test to compare the number of pancreatic β-cells. The results showed that the administration of Klika ongkea extracts affected the pancreatic β-cells regeneration. These findings suggest that Klika ongkea has an effect on the regeneration of pancreatic β-cells in streptozotocin-induced rats.


Keywords : Keywords: Klika Ongkea extract, Pancreatic β-cells, streptozotocin
Full Text: PDF


References


[1] He, L., He, T., Farrar, S., Ji, L., Liu, T and Ma, X, Cell. Physiol. Biochem., 2017, 44 (2), 532–553.

[2] Arman, M. S. I., Al Mahmud, A., Mahmud, H. R and Reza, A. A., Discov. Phytomedicine, 2019, 6 (3), 99–101.

[3] International Diabetes Federation, IDF Diabetes Atlas, 9th ed., 2019, International Diabetes Federation, Brussels, Belgium.

[4] International Diabetes Federation, IDF Diabetes Atlas, 10th ed., 2021, International Diabetes Federation, Brussels, Belgium.

[5] Indonesian Health Ministry, InfoDATIN: Pusat Data dan Informasi Kementerian Kesehatan RI Diabetes Melitus, 2020, Indonesian Health Ministry, Jakarta.

[6] Rifa’i, M., Wahyuningsih, M. D., Lestari, N. D., Ibrahim, M., Soewondo, A and Tsuboi, H, Med. Plants - Int. J. Phytomedicines Relat. Ind., 2018, 10 (4), 305.

[7] Zhu, X., Ouyang, W., Lan, Y., Xiao, H., Tang, L., Liu, G., Feng, K., Zhang, L., Song, M and Cao, Y, Food Biosci., 2020, 35, 100574.

[8] Cory, H., Passarelli, S., Szeto, J., Tamez, M and Mattei, J, Front. Nutr., 2018, 5, 87.

[9] Murdifin, M., Pakki, E., Alam, G., Manggau, M., Muslimin, L., Rusdi, M and Wahyudin, E., Pharmacogn. J., 2017, 9 (2), 171–175.

[10] Sosef, M. S. M., Hong, L. T and Prawirohatmodjo, S, Plant Resources of South-East Asia. No. 5(3): Timber Trees: Lesser-Known Timbers, 1998, Prosea Foundation, Bogor.

[11] Murdifin, M., Wahyudin, E., Lawrence, G. S., Subehan, Manggau, M. A and Alam, G, Pharmacogn. J., 2012, 4 (34), 18–21.

[12] Jangga, J., Natsir, R., As’ad, S and Bukhari, A, Int. J. Sci. Technol. Res., 2015, 4 (4), 307–312.

[13] Oh, Y. S., Evid. Based Complement. Alternat. Med., 2015, 2015, e629863.

[14] Tan, R. T. R., Mohamed, S., Samaneh, G. F., Noordin, M. M., Goh, Y. M and Manap, M. Y. A, Int. Food Res. J., 2011, 18 (1), 179–188.

[15] Khandelwal, P., Khanna, S., Chapter 4 - Diabetic peripheral neuropathy: An insight into the pathophysiology, diagnosis, and therapeutics, in Bagchi, D., Das, A., Roy, S., Eds., Wound Healing, Tissue Repair, and Regeneration in Diabetes, 2020, Academic Press, pp 49–77.

[16] Murnah, M., Pengaruh Ekstrak Etanol Mengkudu (Morinda citrifolia L) terhadap Diabetik Nefropati pada Tikus Spraque Dawley yang Diinduksi Streptozotocin (STZ) dengan Kajian VEGF dan Mikroalbuminuria (MAU) [Master thesis], 2011, Diponegoro University, Semarang, Indonesia.

[17] Ariastuti, R., Fitrawan, L. O. M., Nugroho, A. E and Pramono, S, Indones. J. Pharm., 2020, 31 (4), 312–322.

[18] Abbas, M., Saeed, F., Anjum, F. M., Afzaal, M., Tufail, T., Bashir, M. S., Ishtiaq, A., Hussain, S and Suleria, H. A. R, Int. J. Food Prop., 2017, 20 (8), 1689–1699.

[19] Sandhar, H. K., Kumar, B., Prasher, S., Tiwari, P., Salhan, M and Sharma, P, Int. Pharm. Sci., 2011, 1 (1), 25–41.

[20] Rorsman, P and Ashcroft, F. M, Physiol. Rev., 2018, 98 (1), 117–214.

[21] Adharini, W. I., Nilamsari, R. V., Lestari, N. D., Widodo, N and Rifa’i, M, Pharm. Sci., 2020, 26 (3), 270–278.

[22] Lestari, N. D., Khalleefah, S., Puspitarini, S., Widodo, Soewondo, A and Rifa’i, M, AIP Conf. Proc., 2019, 2155 (1), 020048.

[23] Guz, Y., Nasir, I and Teitelman, G, Endocrinology, 2001, 142 (11), 4956–4968.

[24] Rabinovitch, A., Suarez-Pinzon, W. L., Sorensen, O., Bleackley, R. C and Power, R. F, J. Immunol. Baltim. Md 1950, 1995, 154 (9), 4874–4882.

[25] Lugasi, A., Hóvári, J., Sági, K. V and Bíró, L, Acta Biol. Szeged., 2003, 47 (1–4), 119–125.

[26] Botutihe, B., Efek Ekstrak Rumput Laut Coklat (Sargasum duplicatum Bory) terhadap Profil Radikal Bebas dan Protein Kinase C Paru Tikus (Rattus novegicus) yang Dipapar Benzo[A]piren [Thesis], 2010, Universitas Brawijaya, Malang, Indonesia


Refbacks

  • There are currently no refbacks.


Creative Commons License
This work is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License.