Anti-Inflammatory Activity of an Indole Alkaloid Isolated from Bebuas (Premna serratifolia)
Muhaimin Muhaimin, Muhammad Irhash Shalihin, Madyawati Latief
J. Pure App. Chem. Res. Vol 10, No 2 (2021), pp. 84 - 103
Submitted: February 12, 2021     Accepted: July 20, 2021     Published: July 20, 2021
DOI: http://dx.doi.org/10.21776/ub.jpacr.2021.010.02.584

Abstract



Premna serratifolia known as bebuas has long been used by Malay comunity for both food and traditional medicine. The most notable advantage of this plant is to heal woman after childbirth and to raise the notion that contains anti-inflammatory bioactive compounds. In this study, the hexane extract of bebuas was only contained steroids. Meanwhile the ethanol extract contained various secondary metabolites such as phenolics, alkaloids, flavonoids, steroids and saponins. The ethanol extract was further separated since the targeted compound isolate was an alkaloid. The isolate obtained was a yellow crystal which transformed to yellow oil after being exposed to air. The anti-inflammatory evaluation of the compound gave a result with a strong activity with ED50 = 4.06 mg/KgBW. Characterization by UV-Vis and FT-IR showed that the isolate’s spectra pattern had similarities with bufotenine. It revealed that the isolate shared the same basic skeleton with the bufotenine, especially the indole skeleton. Furthermore, it also shared the same physical form with bufotenine. These evidences strengthened the assumption that the isolate was an indole alkaloid.

Keywords : Premna serratifolia, anti-inflammatory, indole, alkaloid, bufotenine
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References


[1] Saim., Pros. Sem. Nas. dan Lokakarya Etnobotani 1. Cisarua Bogor., 381–389, 1992.

[2] Sudarmono, S., JTLS, 2018, 8 (2), 238115.

[3] Nunes, C.D.R., Barreto Arantes, M., Menezes de Faria Pereira, S., Leandro da Cruz, L., de Souza Passos, M., Pereira de Moraes, L., Vieira, I.J.C and Barros de Oliveira, D., 2020, 25 (16), 3726.

[4] Dianita, R and Jantan, I., Pharm. Biol., 2017, 55 (1), 1715-1739.

[5] Katzung, B.G., Masters, S.B and Trevor, A.J. eds., Basic Clin. Pharmacol, 2004.

[6] Parawansah, P., Nuralifah, N., Akib, N and Antrie, G., Prosiding Seminar Nasional Riset Kuantitatif Terapan, 2017, 1 (1).

[7] Adyttia, A., Untari, E.K and Wahdaningsih, S., Int. J. Pharm. Sci. Res, 2014, 1 (2), p.5.

[8] Singh, C., Anand, S.K., Tiwari, K.N., Mishra, S.K and Kakkar, P., 3 Biotech, 2021, 11 (3), 1-11.

[9] Ikawati, Z., Farmakoterapi Penyakit Sistem Syaraf Pusat, 2011, Bursa Ilmu, Yogyakarta.

[10] Black, P.H and Garbutt, L.D., J. Psychosom. Res, 2002, 52 (1), 1-23.

[11] World Health Expectancy, ‘Indonesia: Life Expectancy’, World Health Expectancy, 2018. [Online]. Available: https://www.worldlifeexpectancy.com/indonesia-life-expectancy. [Accessed: 10-Dec-2019].

[12] Tjay T. H and Rahardja, K, Obat-Obat Penting Khasiat, Penggunaan dan Efek-Efek Sampingnya, 6th ed, 2007, PT. Elex Media Komputindo, Jakarta.

[13] Harbone, J. B, Metode Fitokimia : Penuntun Cara Modern Menganalisis Tumbuhan, 2nd ed. 1987, ITB Press, Bandung.

[14] Titis, M., Fachriyah, E and Kusrini, D, Chem Info J., 2013. 1 (1), 196–201.

[15] Emrizal, A. Fernando, F. Suryani, F. Ahmad, H. M. Sirat and Dayar Arbain, D, J. Penelit. Farm. Indones, 2012, 1 (1), 1–5.

[16] Hasanah, F and Hidayah, N., J. Pharm. Sci, 2018, 1 (1), 16-22.

[17] Apridamayanti, P., Sanera, F and Robiyanto, R., Int. J. Pharm. Sci. Res, 2018, 5 (3), 6.

[18] Luliana, S., Susanti, R and Agustina, E., Traditional Medicine Journal, 2017, 22 (3), 199-205.

[19] Ross G, J, S and Finney, D. J, Stat, 1972, 21 (3), 222.

[20] Pagare, S., Bhatia, M., Tripathi, N., Pagare, S and Bansal, Y.K., Curr. Trends Biotechnol. Pharm, 2015, 9 (3), 293-304.

[21] Aslamiah, S and Haryadi, H., Anterior J, 2014, 14 (1), 11-19.

[22] Williamson, E.M., Okpako, D.T and Evans, F.J., Selection, Preparation and Pharmacological Evaluation of Plant Material, Volume 1, 1996, John Wiley Sons Inc, Hoboken, New Jersey.

[23] Oktaviani, E., Wibowo, M.A and Idiawati, N., Jurnal Kimia Khatulistiwa, 2015, 4 (3).

[24] Dianita, R and Jantan, I., Pharm. Biol, 2017, 55 (1), 1715-1739.

[25] Wibowo, S and Gofir, A, Farmakoterapi Nyeri Inflamasi: Farmakoterapi dalam Neurologi, 1st ed, 2001, Salemba Medika, Jakarta.

[26] Vandamme, T. F, J. Pharm. Bioallied. Sci, 2014, 6 (1) , 2–9.

[27] Amri, O., Zekhnini, A., Bouhaimi, A., Tahrouch, S and Hatimi, A, Pharmacog. J, 2018, 10 (1) , 71-76.

[28] Hussein, R. A and El-Anssary, A. A, Herb. Med, 2019, 2, 11-30.

[29] Sangster, A.W and Stuart, K.L., Chem. Rev, 1965, 65 (1), 69-130.

[30] Ivan, B.C., Caira, M.R and Dumitrascu, F, Rev. Chim, 2020, 71, 51-57.

[31] Mishraki‐Berkowitz, T., Kochelski, E., Kavanagh, P., O'Brien, J., Dunne, C., Talbot, B., Ennis, P and Wolf, U., J. Forensic Sci, 2020, 65 (5), 1450-1457.

[32] Moreira, L. A., Murta, M. M., Gatto, C. C, Fagg, C. W and Dos Santos, M. L, Nat. Prod. Commun, 2015, 10(4), 581–584.

[33] Dey, P., Kundu, A., Kumar, A., Gupta, M., Lee, B. M., Bhakta, T., Dash, S. and Kim, H. S, Analysis of alkaloids (indole alkaloids, isoquinoline alkaloids, tropane alkaloids). In Recent Advances in Natural Products Analysis; Elsevier: Amsterdam, The Netherlands, 505–567. 2020.


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