The Mechanically Extraction Process of Gambier (Uncaria gambier Roxb.) from Limapuluh Kota, West Sumatera and Its Antioxidant activity
The extraction process of leaves and twigs from gambier (Uncaria gambier Roxb) was conducted mechanically by using traditionally hydraulic press, conventional screw press, and modified twin-screw press. The leaves and twigs of gambier was obtained from Limapuluh Kota, West Sumatera, Indonesia. The water, ash and catechin contents of the gambier extracts were analyzed based on gambier’s SNI, SNI 01-3391-2000 using spectrophotometry and thermogravimetry method. Antioxidant activity analysis of the extracts was also performed by 1,1-diphenyl-2-pikrilhidrazyl (DPPH) method and compared to vitamin C as a standard antioxidant. The analysis results showed that the extracts contain no ash. Meanwhile, the catechin and water contents of the extracts were approximately 50 and 13% thus the extracts were classified as quality gambier 2. Other than that, analysis result of catechin and epicatechin contents of the extracts using HPLC and compared to the reference materials showed that catechins and epicatechins contents of the extracts around 5.77-18.3X102 and 6.29-8.89X105 ppm. The antioxidant activity of the extracts were 2.5 times stronger than vitamin C with IC50 value of 4.37-4.52 µg/mL so that the extracts were categorized as very active as an antioxidant.
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