Characterization of the Curcuminoids Fingerprint Profile in Curcuma and Zingiber Genera by TLC – Digital Image Analysis

Authors

  • Anisa Lailatusy Syarifah Brawijaya University
  • Rurini Retnowati Brawijaya University
  • Hermin Sulistyarti Brawijaya University

DOI:

https://doi.org/10.21776/ub.jpacr.2019.008.02.462

Keywords:

curcuma, zingiber, curcuminoid, thin-layer chromatography, grey value, RGB

Abstract

Curcuma longa, C. xanthorrhiza, C. heyneana, and Zingiber cassumunar contain high curcuminoid and have relatively the similar yellow color. Therefore, that they are potentially adulterated and difficult to differentiate in the form of powder. Hence, it is necessary to characterize the fingerprints compound profile by a simple and rapid method. This research aims to determine fingerprint compound profile of curcuminoid using Thin Layer Chromatography (TLC) and digital image analysis. The result of the research identified that the fingerprint compound profile of curcuminoid on the four rhizomes was obtained by TLC method using silica gel 60 GF254 as the stationary phase, chloroform:dichloromethane:methanol (13:6:1) as the mobile phase, and observation under UV 254 nm light and citroborate reagent. Thereafter, the digital image analysis was carried out using Image J software according to the gray value and % of RGB (red-green-blue) value. Based on gray value and % of RGB, both Curcuma and Zingiber genera were differentiated through curcumin compound (Rf 0.63), demethoxycurcumin (Rf 0.34), bisdemethoxycurcumin (Rf 0.21). The profile of fingerprint compound on Curcuma longa, C. xanthorrhiza, C. heyneana, and Zingiber cassumunar was differentiated through Rf 0.26; Rf 0.17; and Rf 0.10.

 

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Author Biography

  • Anisa Lailatusy Syarifah, Brawijaya University
    Chemistry Department

References

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Published

2019-06-12

How to Cite

Characterization of the Curcuminoids Fingerprint Profile in Curcuma and Zingiber Genera by TLC – Digital Image Analysis. (2019). The Journal of Pure and Applied Chemistry Research, 8(2), 147-161. https://doi.org/10.21776/ub.jpacr.2019.008.02.462