Determination of Radiochemical Purity of 99mTc-DTPA Using One-System Method of Paper Chromatography

Authors

  • Maskur Maskur Center for Radioisotope and Radiopharmaceutical Technology, National Nuclear Energy Agency (BATAN)
  • Enny Lestari Center for Radioisotope and Radiopharmaceutical Technology, National Nuclear Energy Agency (BATAN)
  • Amal Rezka Putra Center for Radioisotope and Radiopharmaceutical Technology, National Nuclear Energy Agency (BATAN)
  • Dede Kurniasih Center for Radioisotope and Radiopharmaceutical Technology, National Nuclear Energy Agency (BATAN)
  • Endang Sarmini Center for Radioisotope and Radiopharmaceutical Technology, National Nuclear Energy Agency (BATAN)
  • Yayan Tahyan Center for Radioisotope and Radiopharmaceutical Technology, National Nuclear Energy Agency (BATAN)
  • Adang Hardi Gunawan Center for Radioisotope and Radiopharmaceutical Technology, National Nuclear Energy Agency (BATAN)
  • Arni Aries Center for Radioisotope and Radiopharmaceutical Technology, National Nuclear Energy Agency (BATAN)

DOI:

https://doi.org/10.21776/ub.jpacr.2019.008.02.461

Keywords:

Radiochemical purity, 99mTc-DTPA, 99mTc, one-system of TLC, two-system of TLC

Abstract

The more efficient and effective quality control techniques for 99mTc-DTPA are needed because 99mTc has a short half-life of around 6.0 hours. We have succeeded in developing a one-system of TLC for radiochemical purity testing system that is faster and more practical. Two-system method of TLC for radiochemical purity testing uses mobile phase of methyl ethyl ketone as an A and 0.9% sodium chloride solution as a B. One-system method uses the mobile phase of a mixture solution of acetone and 0.9% sodium chloride. In this study, the determination of radiochemical purity of the one-system of TLC has been successfully developed using the Whatman-1 paper stationary phase and the mixed mobile phase between acetone and 0.9% sodium chloride solution. The mobile phase of acetone: 0.9% sodium chloride with a ratio of 9:1 shows the most optimum results. This phase can separate 99mTc-DTPA (Rf = 0.4-0.6) from 99mTcO4- (Rf = 0.9-1.0) and 99mTcO2 (Rf = 0.0-0.1) as radiochemical impurities. This result shows that the one-system of TLC method can be used for radiochemical purity testing of 99mTc-DTPA radiopharmaceutical kits. This method can completely separate the product compound (99mTc-DTPA) from its impurities (99mTcO2 and 99mTcO4-).

Downloads

Download data is not yet available.

References

[1] Fitri Imelda, Endang Susalit, M Bonar M Marbun, C. M. R., J. Penyakit Dalam Indonesia, 2017, 4, 128–136.

[2] Maskur, Purwoko, Chairuman, Yono S., Pros. Semin. Nas. Kim. FMIPA Univ. Negeri Surabaya, 2015, 11–18.

[3] Stefanus Santosa, Agus Widjanarko, C. S., J. Pseudocode III, 2016, 163–170.

[4] Sevin Ayaz. J. Clin. Anal. Med., 2017, 8, 8–11.

[5] Abdel, M. & Hamed, G. Iran. J. Nucl. Med., 2017, 25 (1), 17–22.

[6] Piepsz, A., Eur. J. Radiol., 2002, 43, 146–153 (2002).

[7] Delima, E. T. et al. Bul. Penelit. Kesehat., 2014, 45, 17–26.

[8] Levey, A. S. & Coresh, J. Lancet, 2012, 379, 165–180.

[9] Wiguno Prodjosudjadi, A. S., J. Ethn. Dis., 2009, 19, 33–36.

[10] Williams, J.K.Y., Nurs. Clin. NA, 2017, 52, 575–587.

[11] Serdar Savas SGul, Zekiye Hasbek, Taner Erselcan, Melih Akyol, B. T. Acta Medica Mediter., 2015, 31, 1247–1252.

[12] Kim, Y., Ha, S., So, Y. & Lee, W. W. Eur Radiol., 2014, 24, 413–422.

[13] Razaq, T., Nisar, H., Roohi, S., Shehzad, A. & Ahmad, I. Iran J Nucl Med, 2017, 25, 122–128.

[14] Taylor, A. T. J. Nucl. Med., 2014, 55, 1–9.

[15] Maskur, Enny Lestari, Endang Sarmini, Yayan Tahyan, Amal Rezka Putra, Dede Kurniasih, A. H. G. Urania, 2017, 23, 57–68.

[16] Sadeghpour, H., Alavi, M., Shahedi, M., Entezarmahdi, S. M. & Sakhteman, A., Trends Pharm. Sci., 2015, 1, 15–19.

[17] Shankar Vallabhajosula, Ronan P. Killeen, and J. R. O. YSNUC, 2010, 40, 220–241.

[18] Zimmerman, B. E., Herbst, C., Norenberg, J. P. & Woods, M. J. Appl. Radiat. Isot., 2006, 64, 1142–1146.

[19] Millar, A. M., Brien, Ã. L. M. O., Beattie, L. A. & Craig, F. J. Label Compd. Radiopharm., 2010, 53, 11–14.

[20] Leonardi, N. M., Casale, G. A., Nicolini, J., Zubata, P. D. & Zubillaga, M. B. J. Nucl. Med. Technol., 2015, 40, 271–275.

[21] Candela, M., Tesán, F. C., Leonardi, N. M., Zubillaga, M. B. & Salgueiro, M. J. Appl. Radiat. Isot., 2013, 82, 322–324.

[22] Korkmaz, M. & Özer, A. Y. J. Pharm. Sci., 1996, 22, 105–110.

[23] Maioli, C., Lucignani, G., Strinchini, A., Tagliabue, L. & Del Sole, A. Acta Biomed., 2017, 88, 49–56.

Downloads

Published

2019-06-12

Issue

Vol. 8 No. 2 (2019): Edition May-August 2019

Section

Articles

License

Authors who publish with this journal agree to the following terms:

  1. Authors retain copyright and grant the journal right of first publication with the work simultaneously licensed under a Creative Commons Attribution-NonCommercial 4.0 International License that allows others to share the work with an acknowledgement of the work's authorship and initial publication in this journal.
  2. Authors are able to enter into separate, additional contractual arrangements for the non-exclusive distribution of the journal's published version of the work (e.g., post it to an institutional repository or publish it in a book), with an acknowledgement of its initial publication in this journal.
  3. Authors are permitted and encouraged to post their work online (e.g., in institutional repositories or on their website) prior to and during the submission process, as it can lead to productive exchanges, as well as earlier and greater citation of published work (See The Effect of Open Access).

How to Cite

Determination of Radiochemical Purity of 99mTc-DTPA Using One-System Method of Paper Chromatography. (2019). The Journal of Pure and Applied Chemistry Research, 8(2), 109-116. https://doi.org/10.21776/ub.jpacr.2019.008.02.461