Modulation of Perlecan Protein towards Chondrocyte Secretion Factors at the Articular Cartilage in Hyperglycemic Animal Model
Ibrahim Njoto, Fatchiyah Fatchiyah, Kusworini Handono, Abdurrachman Abdurrachman, Djoko W. Soeatmadji, Handono Kalim
J. Pure App. Chem. Res. Vol 8, No 1 (2019), pp. 80-86
Submitted: January 04, 2019     Accepted: April 18, 2019     Published: April 18, 2019


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High carbohydrate influences the cartilage microcellular environment and chondrocytes. Perlecan (HSPG2) conducts an essential role as chondrocytes mechano-transducer and chondrocytes secretion factors. This research aims to fulfill the research gap about hyperglycemia which influences to perlecan of articular cartilage. About twenty male rats were divided into four groups: group I (sugar-treated rats 1.00 mg/kg of BW), group II (sugar-treated rats 1.5 mg/kg of BW), group III (2.00 mg/kg of BW), and control. The Anterior Cruciate Ligament Transection (ACLT) used to generate an unstable joint to be osteoarthritis condition at the right knee. Then, sugar was administrated for two months.  Level expression of the protein was analyzed using Western Blotting. The result showed that perlecan expression was decreased within all sugar-treated rats group as compared to control. The IGF-1 expression was decreased, whereas TGF-β was slightly increased within all treatment groups. Moreover, the expression of FGF-2 was higher in all treatment groups. Of note, IL-1β expression was only elevated in group II and III. The interaction of perlecan to the chondrocyte secretion factors was determined by the cartilage condition within hyperglycemia.

Keywords : anabolic, catabolic, chondrocyte, hyperglycemia, perlecan
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[1] Vincent TL, McLean CJ, Full LE, Peston D, Saklatvala J. Osteoarthr. Cartilage2007, 15(7):752-763.

[2] LeBleu VS, MacDonald B, Kalluri R. Exp. Biol. Med.2008, 232(9):1121-1129.

[3] Kaneko H, Ishijimaa M, Futamia I, Tomikawa-Ichikawa N, Kosaki K, Sadatsukia R, Yamada Y, Kurosawa H, Kaneko K, Hirasawa-Arikawa E. Matrix Biol.2013, 32:1-19.

[4] Sadatsuki R, Kaneko H, Futami I, Hada S, Culley KL, Otero M, Dragomir C, Kinoshita M, Goldring MB, Yamada Y. Osteoarthr. Cartilage, 2014, 22: S57-S489.

[5] Melrose J, Smith MM, Smith SM, Ravi V, Young AA. Dart AJ, Sonnabend DH, Little CB. Tissue Cell. 2013, 45:77– 82.

[6] Pacifici M, Koyama E, Iwamoto M. Birth Defects Res. C Embryo Today,2005, 75:237-248.

[7] Wang J, Kramer WC, Schroeppel JP. Transcriptional regulation of articular chondrocyte Function and its implication in osteoarthritis. Principles of osteoarthritis– Its Definition, Character, Derivation and Modality-Related Recognition, IntechOpen, 2012; 474-488.

[8] Chaterine A, Vorgl-Willis, Iris J, Edwards. Biochim.t Biophys. Acta.2004, 1672:36-45.

[9] Steenvoorden MMMC, Huizinga TWJ, Verzijl N, Bank RA, Ronday HK, Luning HAF, Lafeber FPJG, Toes RM, DeGroot JD. Arthritis Rheum.2006, 54(1):253–263.

[10] Rosa SC, Rufino AT, Judas FM, Tenreiro, C. M., Lopes, M. C., and Mendes, A. F. J Cell Biochem. 2011, 112:2813–2814.

[11] Kraeutler MJ, Wolsky RM, Vidal AF, Bravman JT. J. Bone Joint Surg.,2017, 99:438-445.

[12] Iqbal K. Morphol. Sci,2012, 29(1):8-11.

[13] Gubbiotti MA, Neill T, Iozzo RV. Matrix Biol.,2016, 1292:14-28.

[14] Chaterine A, Vorgl-Willis, Iris J, Edwards.: Biochim. Biophys. Acta,2004, 1672:36-45.

[15] Yayon A, Klagsburn M, Esko JD, Leder P, Ornitz DM. Cell,1991, 64:841-848.

[16] Nugent MA, Edelman ER. Biochemistry.1992, 31:8876-8883.

[17] Forsten KE, Courant NA, Nugent MA. J. Cell. Physiol.1997, 172:209-220.

[18] Nugent MA, Iozzo RV. Int. J. Biochem. Cell Biol.2000, 32:115-120.

[19] Wilusz RE, Defrate LE, Guilak F. A. Matrix Biol.,2012, 31(6):320-327.

[20] Yaeger PC, Masi TL, de Ortiz JL, Binette F, Tubo R, McPherson JM. Exp. Cell Res.1997, 237: 318–325.

[21] Yang X, Chen L, Xu X, Li C, Huang C, Deng CX. J. Cell Biol.,2001, 153:35–46.

[22] Yan D, Chen D, Cool SM, van Wejnen AJ, Mikecz K, Murphy G, Im H-J. Arthritis Res. Ther.,2011, 13: R130.

[23] Forsten KE, Courant NA, Nugent MA. J. Cell Physiol.,1997, 172:209-220.


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