Influence of Treating Essential Oils of Canarium Tree Resin on The Level Malondialdehyde (MDA), Protease Activity, Bronchi Histopathology Description and Expression of Tumor Necrosis Alpha Factor (TNF-α) on Asthma Rats (Rattus norvegicus)

Authors

  • Faijal Fachrudin Mbabho Department of Chemistry, Faculty of Mathematics and Science, Brawijaya University
  • Aulanni’am Aulanni’am Department of Biochemistry, Faculty of Veterinary Medicine, Brawijaya University
  • Arie Srihardyastutie Department of Chemistry, Faculty of Mathematics and Science, Brawijaya University

DOI:

https://doi.org/10.21776/ub.jpacr.2018.007.03.427

Keywords:

canarium tree resin, asthma, essential oils

Abstract

Essential oils of canarium tree resin (Canarium indicum L) have anti-inflammatory and antioxidant activity. Inflammation of the bronchi and airway remodeling is a major factor in the occurrence of asthma. The purpose of this study was to study the potential of essential oils of canarium tree resin on the expression of TNF-α, changes in bronchial epithelial cell features, MDA levels and protease activity from OVA and LPS exposure in a rat model of asthma. This study used four groups of rat, the negative control group, asthma group, therapy group dose 25 mg/kg BB and 50 mg/kg BB. TNF-α expression was calculated using Walter calculations, histopathologic observation of bronchi was observed using Olympus BX51 microscope, MDA level was measured using Thiobarbituric Acid (TBA) method and measurement of protease activity was done by spectrophotometric method, and analyzed by ANOVA. The results showed that the therapy of essential oils of canarium tree resin at 25 mg/kg BB and 50 mg/kg BB significantly (P<0.05), after treatment for seven days decreased, MDA 44%, Protease activity 46%, improved bronchial epithelium and TNF-α expression by 51%. This study proves that essential oils of canarium tree resin can be effectively used as therapy in asthma model rats.

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References

[1] Wei, D.-Z., Guo, X.-Y., Lin, L.-N., Lin, M.-X., Gong, Y.-Q., Ying, B.-Y., Huang, M.-Y. Inflammation 2016, 39 (6), 1876–1882.

[2] Babu, S. K., Puddicombe, S. M., Arshad, H. H., Wilson, S. J., Ward, J., Gozzard, N., Higgs, G., Holgate, S. T., Davies, D. E. Clin. Immunol. 2011, 140 (1), 18–25.

[3] Kumari, A., Dash, D., Singh, R. Cytokine 2015, 76 (2), 334–342.

[4] Abu Bakar, N., Anyanji, V. U., Mustapha, N. M., Lim, S.-L., Mohamed, S. J. Funct. Foods 2015, 19, 710–722.

[5] Varghese, A., Ticktin, T. Ecol. Soc. 2008, 13 (2).

[6] Thang, T., Dai, D., X Luong, N., Ogunwande, I. Nat. Prod. Res. 2014, 28.

[7] Muthuswamy, R. Iran. J. Pharm. Sci. 2014, 9, 13–21.

[8] Ueno-Iio, T., Shibakura, M., Yokota, K., Aoe, M., Hyoda, T., Shinohata, R., Kanehiro, A., Tanimoto, M., Kataoka, M. Life Sci. 2014, 108 (2), 109–115.

[9] Utomo, H. J. Dent. Indones. 2013, 19.

[10] Nagawa, C., Böhmdorfer, S., Rosenau, T. Ind. Crops Prod. 2015, 71, 75–79.

[11] Gaballah, H. H., Gaber, R. A., Sharshar, R. S., Elshweikh, S. A. Gene 2018, 660, 128–135.

[12] Lee Jeâ€Hyuk, Chang Kyungâ€Mi, Kim Gunâ€Hee. J. Sci. Food Agric. 2009, 89 (10), 1762–1769.

[13] Alina, C.-M. C., Rocío, R.-L., Aurelio, R.-M. M., Margarita, C.-M. M., Román-Guerrero, A., Rubén, J.-A. J. Essent. Oil Bear. Plants 2014, 17 (5), 758–768.

[14] Lin, J.-J., Lin, J.-H., Hsu, S.-C., Weng, S.-W., Huang, Y.-P., Tang, N.-Y., Lin, J.-G., Chung, J.-G. Vivo Athens Greece 2013, 27 (6), 809–814.

[15] Reddy, P. H. Pharm. Basel Switz. 2011, 4 (3), 429–456.

[16] Huang, W.-C., Fang, L.-W., Liou, C.-J. Front. Immunol. 2017, 8, 134.

[17] Yoon, W.-J., Lee, N. H., Hyun, C.-G. J. Oleo Sci. 2010, 59 (8), 415–421.

[18] Hocaoglu, A. B., Karaman, O., Erge, D. O., Erbil, G., Yilmaz, O., Kivcak, B., Bagriyanik, H. A., Uzuner, N. Iran. J. Allergy Asthma Immunol. 2012, 11 (4), 316–323.

[19] de Cássia da Silveira e Sá, R., Andrade, L. N., de Sousa, D. P. Mol. Basel Switz. 2013, 18 (1), 1227–1254.

[20] Lima, D. F., Brandão, M. S., Moura, J. B., Leitão, J. M. R. S., Carvalho, F. A. A., Miúra, L. M. C. V., Leite, J. R. S. A., Sousa, D. P., Almeida, F. R. C. J. Pharm. Pharmacol. 2012, 64 (2), 283–292.

[21] Dheeb, B. I. BMC Genomics 2014, 15 (Suppl 2), P71.

[22] Dumont, N., Lepage, K., Côté, C. H., Frenette, J. J. Appl. Physiol. Bethesda Md 1985 2007, 103 (1), 97–104.

[23] Siqueira, H. D. S., Neto, B. S., Sousa, D. P., Gomes, B. S., da Silva, F. V., Cunha, F. V. M., Wanderley, C. W. S., Pinheiro, G., Cândido, A. G. F., Wong, D. V. T., Ribeiro, R. A., Lima-Júnior, R. C. P., Oliveira, F. A. Life Sci. 2016, 160, 27–33.

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Published

2018-09-12

Issue

Vol. 7 No. 3 (2018): Edition September-December 2018

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Articles

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How to Cite

Influence of Treating Essential Oils of Canarium Tree Resin on The Level Malondialdehyde (MDA), Protease Activity, Bronchi Histopathology Description and Expression of Tumor Necrosis Alpha Factor (TNF-α) on Asthma Rats (Rattus norvegicus). (2018). The Journal of Pure and Applied Chemistry Research, 7(3), 282-291. https://doi.org/10.21776/ub.jpacr.2018.007.03.427