Green Tea Extract (Camellia sinensis L.) Effects on Uric Acid Levels on Hyperuricemia Rats (Rattus norvegicus)

Authors

  • Putranty Widha Nugraheni Chemistry Department Brawijaya University
  • Fitria Rahmawati Chemistry Department Brawijaya University
  • Chanif Mahdi Chemistry Department Brawijaya University
  • Sasangka Prasetyawan Chemistry Department Brawijaya University

DOI:

https://doi.org/10.21776/ub.jpacr.2017.006.03.355

Keywords:

hyperuricemia, uric acid, green tea extract, Camellia sinensis, uric acid levels

Abstract

Uric acid is the end product of purine degradation. When uric acid levels exceed normal limits, it will build up and cause hyperuricemia. Allopurinol is one of the most effective and common medicine for hyperuricemia, but it brings serious side effects, therefore it is needed alternative therapy for hyperuricemia. One plant that may be expected to low uric acid levels is green tea (Camellia sinensis L.), that contains many antioxidants polyphenols, especially flavonoids. Flavonoid has strong antioxidant properties, act as free radical and metal scavengers, and also xanthine oxidase (XOD) inhibitors. This study investigates the potential of green tea using various doses of 150 mg/kg, 300 mg/kg, and 600 mg/kg of body weight in 24 white male rats (Rattus norvegicus) Wistar strain that has been received high purine diet in 60 consecutive days. This study used DHBSA methods to measure uric acid levels in blood serum and urine that excreted 8 hours before surgery. Green tea extract that contains polyphenol can inhibit XOD activities, therefore, it leads to decrease uric acid level in blood and increase the excretion through urine by modulating urate gene transporter. A therapy with 600 mg/kg body weight of GTE is the most effective dose to decrease uric acid levels in serum and to increase excretion of exceeding uric acid significantly (p < 0.01), from One Way ANOVA and Tukey analysis.

Downloads

Download data is not yet available.

References

[1] G. Lippi, M. Montagnana, M. Franchini, E. J. Favaloro, and G. Targher, Clin. Chim. Acta, 2008, 392(1–2), 1–7.

[2] L. A. Baldree M. D. and F. B. Stapleton M. D., Pediatr. Clin. North Am., 1990, 37(2), 391-418.

[3] D.-H. Kang, T. Nakagawa, L. Feng, S. Watanabe, L. Han, M. Mazzali, L. Truong, R. Harris and R. J. Johnson, J. Am. Soc. Nephrol., 2002, 13(12), 2888–2897.

[4] M. E. Balis, Uric Acid Metabolism in Man. 1984, The Memorial Sloan-Kettering Cancer Center, New York.

[5] P. Cirillo, W. Sato, S. Reungjui, M. Heinig, M. Gersch, Y. Sautin, T. Nakagawa and R. J. Johnson, J. Am. Soc. Nephrol., 2006, 17(12), S165-S168.

[6] H. M. Kramer and G. Curhan, Am. J. Kidney Dis., 2002, 40, 1, 37–42.

[7[ A. N. Rohma, C. Mahdi, and A. Aulanni’am, J. Pure Appl. Chem. Res., 2017, 6(2), 160–165.

[8] D. I. Feig and R. J. Johnson, Hypertension, 2003, 42(3), 247–252.

[9] D. I. Feig, B. Soletsky, and R. J. Johnson, JAMA, 2008, 300(8), 924–32.

[10] D. Astuti, Efek Antihiperurisemia Kombinasi Ekstrak Air Kelopak Rosella (Hibiscus sabdariffa L.) dan Akar Tanaman Akar Kucing (Acalypha indica L.) Pada Tikus Putih Jantan yang Diinduksi Putih Jantan yang Diinduksi Kalium Oksonat, Department of Pharmacy, University of Indonesia, Indonesia, 2011.

[11] H. K. Choi and G. Curhan, Arthritis Rheum., 2007, 57(5), 816–821.

[12] C. G. Fraga, M. Galleano, S. V. Verstraeten, and P. I. Oteiza, Mol. Aspects Med., 2010, 31(6), 435–445.

[13] G. Chen, M. L. Tan, K. K. Li, P. C. Leung, and C. H. Ko, J. Ethnopharmacol., 2015, 175, 14–20.

[14] D. Bolignano M.D., G. Coppolino M.D., A. Barillà M.D., S. Campo M.D., M. Criseo M.D., D. Tripodo M.D., M. Buemi M.D., J. Ren. Nutr., 2007, 17(4), 225–234.

[15] P. J. Rogers, J. E. Smith, S. V Heatherley, and C. W. Pleydell-Pearce, Psychopharmacology (Berl)., 2008, 195, 569–577.

[16] T. Kakuda, A. Nozawa, T. Unno, N. Okamura, and O. Okai, Biosci. Biotechnol. Biochem., 2000, 64, 2, 287–293.

[17] K. Kaneko, Y. Aoyagi, T. Fukuuchi, K. Inazawa, and N. Yamaoka, Biol. Pharm. Bull., 2014, 37(5), 709–721.

[18] D. Brulé, G. Sarwar, and L. Savoiet, J. Food Compos. Anal., 1988, 1(2), 130–138.

[19] H. K. Choi, S. Liu, and G. Curhan, Arthritis Rheum., 2005. 52, 1, 283–289.

[20] M. H. O’Regan, J. W. Phillis, and G. A. Walter, Neurochem. Int., 1989, 14, 1, 91–99.

[21] K. Jatuworapruk, S. Srichairatanakool, S. Ounjaijean, N. Kasitanon, S. Wangkaew, and W. Louthrenoo, J. Clin. Rheumatol., 2014, 20, 6, 310–3.

[22] A. Rashidinejad, E. J. Birch, and D. W. Everett, J. Food Compos. Anal., 2016, 48, 120–127.

[23] K. Boudiaf, Z. Houcher, S. Widad, and M. Benboubetra, JABS, 2010, 4(1), 7–16.

[24] T. Hayashi, K. Sawa, M. Kawasaki, M. Arisawa, M. Shimizu, and N. Morita, J. Nat. Prod., 1988, 51(2), 345–348.

[25] F. Preitner, A. Laverriere-Loss, S. Metref, A. D. Costa, C. Moret, S. Rotman, D. Bazin, M. Daudon, C. Sandt, A. Dessombz, B. Thorens, Am. J. Physiol. Renal Physiol., 2013, 305, 5, F786-95.

[26] M. S. Lipkowitz, Curr. Rheumatol. Rep., 2012, 14(2), 179–188.

[27] X.Wu, D. M. Muzny, C. C. Lee, and C. T. Caskey, J. Mol. Evol., 1992. 34(1), 78–84.

[28] P. H. F. Gois, D. Canale, R. A. Volpini, D. Ferreira, M. M. Veras, V. Andrade-Oliveira, N. O. S. Câmara, M. H. M. Shimizu, A. C. Seguro, Free Radic. Biol. Med., 2016, 101, 176–189.

[29] R. A. Shaw, S. Kotowich, H. H. Mantsch, and M. Leroux, Clin. Biochem., 1996, 29, 1, 11–19.

[30] A. Enomoto, H. Kimura, and A. Chairoungdua, Nature, 2002, 417, 447–452.

[31] K. Ichida, H. Matsuo, T. Takada, A. Nakayama, K. Murakami, T. Shimizu, Y. Yamanashi, H. Kasuga, H. Nakashima, T. Nakamura, Y. Takada, Y. Kawamura, H. Inoue, C. Okada, Y. Utsumi, Y. Ikebuchi, K. Ito, M. Nakamura, Y. Shinohara, M. Hosoyamada, Y. Sakurai, N. Shinomiya, T. Hosoya, and H. Suzuki, Nat. Commun., 2012, 3, 764, 1-7.

[32] A. So and B. Thorens, J. Clin. Invest., 2010, 120, 6, 1791–1799.

Downloads

Published

2017-09-26

Issue

Vol. 6 No. 3 (2017): Edition of September - December 2017

Section

Articles

License

Authors who publish with this journal agree to the following terms:

  1. Authors retain copyright and grant the journal right of first publication with the work simultaneously licensed under a Creative Commons Attribution-NonCommercial 4.0 International License that allows others to share the work with an acknowledgement of the work's authorship and initial publication in this journal.
  2. Authors are able to enter into separate, additional contractual arrangements for the non-exclusive distribution of the journal's published version of the work (e.g., post it to an institutional repository or publish it in a book), with an acknowledgement of its initial publication in this journal.
  3. Authors are permitted and encouraged to post their work online (e.g., in institutional repositories or on their website) prior to and during the submission process, as it can lead to productive exchanges, as well as earlier and greater citation of published work (See The Effect of Open Access).

How to Cite

Green Tea Extract (Camellia sinensis L.) Effects on Uric Acid Levels on Hyperuricemia Rats (Rattus norvegicus). (2017). The Journal of Pure and Applied Chemistry Research, 6(3), 246-254. https://doi.org/10.21776/ub.jpacr.2017.006.03.355