Study of Antocyanins Activity from Purple Sweet Potato for Reducing Apoptotic Cells Expression of The Cerebellum On Ischemic Stroke Rats

Authors

  • Latifah Tribuana Dewi Department of Chemistry, Faculty of Math and Science, Brawijaya University
  • Made Oka Adnyana Department of Chemistry, Faculty of Math and Science, Brawijaya University
  • Chanif Mahdi Department of Chemistry, Faculty of Math and Science, Brawijaya University
  • Sasangka Prasetyawan Department of Chemistry, Faculty of Math and Science, Brawijaya University
  • Arie Srihardyastutie Department of Chemistry, Faculty of Math and Science, Brawijaya University
  • Aulanni’am Aulanni’am Department of Chemistry, Faculty of Math and Science, Brawijaya University Department of Veterinary, Faculty of Veterinary Medicine, Brawijaya University

DOI:

https://doi.org/10.21776/ub.jpacr.2018.007.02.348

Keywords:

Anthocyanin, Purple Sweet Potato, Ischemic Stroke, MCAO, Apoptotic cells.

Abstract

This study aims to determine anthocyanin antioxidant performance with a dose of 2 cc/day based on the expression of apoptotic cells on ischemic stroke rats cerebellum. Research was conducted using ischemic stroke rats (Rattus norvegicus) that prepared by ligated for 3 h on the Common Carotid Artery (CCA) and External Carotid Artery (ECA) followed by reperfusion that commonly known as MCAO (Middle Cerebral Artery Occlusion). The anthocyanin extract was characterized by LC-MS and its IC50 was measured by DPPH method. The rats were divided into five groups 1) negative control; 2) reperfusion 1 h; 3) reperfusion 72 h; 4) reperfusion 24 h, with anthocyanin therapy; 5) reperfusion 72 h, with anthocyanin therapy. The results of LC-MS showed that anthocyanin from purple sweet potato extracts contained Petunidin-3,5-O-diglucoside (Pt-DG) with an IC50 value of 22.16 μg/mL, categorized as very strong antioxidant. The results showed that apoptotic cells expression of cerebellum decreased significantly (p<0.01) after 72 h reperfusion with anthocyanins therapy until 2.42%. The current work proved that anthocyanin extract effectively suppresses the apoptotic cell’s expression of the cerebellum on stroke ischemic rats.

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References

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Published

2018-05-05

Issue

Vol. 7 No. 2 (2018): Edition May-August 2018

Section

Articles

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How to Cite

Study of Antocyanins Activity from Purple Sweet Potato for Reducing Apoptotic Cells Expression of The Cerebellum On Ischemic Stroke Rats. (2018). The Journal of Pure and Applied Chemistry Research, 7(2), 94-99. https://doi.org/10.21776/ub.jpacr.2018.007.02.348