Phytochemical screening, investigation of the antisalmonella, antishigella, and antioxidant properties of the leaves of Rauvolfia vomitoria Afzel
Bénédicta Kpadonou-Kpoviessi, Amandine Noudamadjo, Basile Goueti, Bardieu Atchade, Paul Djouonzo Toukam, Joachim Gbenou, Bienvenu Glinma, Salomé Dossou Sika KPOVIESSI
J. Pure App. Chem. Res. Vol 13, No 1 (2024), pp. 63-71
Submitted: October 13, 2023     Accepted: April 05, 2024     Published: May 06, 2024


Cover Image

Rauvolfia vomitoria is a plant acclimatized in Benin - Africa that people use to treat several illnesses such as malaria, cough, typhoid fever, madness, toothache, diabetes, and nausea. The most used organs are leaves, stems, and roots. Thus, the purpose of this work is to contribute to the valorization of R. vomitoria by chemical and biological studies of this plant. Phytochemical analyses of the powder extracts were performed and revealed the presence of alkaloids, tannins, flavonoids, anthocyanins, saponins, terpenes, coumarins, mucilages, reducing compounds, free anthracene derivatives, bound anthracene derivatives (O-glycosides and C-glycosides) with absence of leuco-anthocyanins, quinone derivatives, steroids and cardiotonic glycosids. Evaluation of antisalmonella and antishigella properties of ethanol-water (70:30; V/V) and ethanolic extracts of R. vomitoria leaves showed minimum inhibitory concentrations (MIC) greater than 2000 µg.mL-1 against Salmonella and Shigella strains. The ethanolic extract was the most active by ABTS and FRAP methods and had very good antioxidant activity at scavenging concentration (SC50) equal to 138,62 µg.mL-1 and 185,99 µg.mL-1 respectively

Keywords : Antioxidant, antisalmonella, antishigella, leaves, Rauvolfia vomitoria
Full Text: PDF


[1] J. T. C. Codjia, A. E. Assogbadjo, and M. R. M. Ekué, Cahiers agricultures, 2003, 12 (5), 321–331

[2] C. Chuberre, Les microalgues: nouvelles sources de molecules elicitrices la sante et la defense des plantes, Biologie vegetale, Normandie Universite, France, 2019

[3] W. Birhan, M. Giday, and T. Teklehaymanot, J. Ethnobiol. Ethnomed., 2011, 7(39), 22132758

[4] S. Kpoviessi, J. Bero, P. Agbani, F. Gbaguidi, B. Kpadonou-Kpoviessi, B. Sinsin, G. Accrombessi, M. Frédérich, M. Moudachirou, and J. Quetin-Leclercq, J. Ethnopharmacol. 2014; 151 (1), 652–659

[5] P. De Carli, 2023, suivi en vie reelle des patiens sous kaftrio, 24ème Colloque Français des Jeunes Chercheurs en mucoviscidose, Présentations orales, Paris:30-31 Maret 2023

[6] L. Pull, X. Bellettre, J. F. Michel, O. Bouchaud, and J. Y. Siriez, Archives de Pédiatrie, 2013, 20 (11), 1260–1264

[7] D. A. E. Zialé, K. C. C. N’gaman-Kouassi, J. Deschamp, N. Bouchemal, T.L. Palama, M. Lecouvey, J. A. Mamyrbekova-Békro, and BékroY-A. J. Pharmacognos. Phytochem. 2023, 12 (1), 06–14

[8] H. O. Ekechi, A. O. Ikwuka, F. C. Udeh, and J. C. Abraham, British J. Med.Heal. Res. 2023, 10 (05), 2394-2967

[9] S. Kumar and B. Singh, Nat. Prod. J., 2023, 2 (1), 303–312

[10] P. J. Houghton and A. Raman, Laboratory handbook for the fractionation of natural extracts. Ed. Chapman and Hall, 1998, Springer, New York

[11] B. Goueti, B. Kpadonou-Kpoviessi, R. Fatondji, B. Atchade, P. D. Toukam, S. Kpoviessi, J. Pure App. Chem. Res., 2023, 12 (2), 57-64

[12] E. Bassene, Initiation à la Recherche sur les Substances Naturelles : Extraction Analyse-Essais Biologiques. Presse Universitaire de Dakar 2012, 94–96, 140

[13] Z. Sroka, G. Zgo, B. Zbikowska, A. Sowa, R. Franiczek, K. Wychowaniec, and B. Krzyzanowska, Microb. Drug Resist., 2019, 25 (04), 0149-0161

[14] N. Bernaert, D. De Paepe, C. Bouten, H. De Clercq, D. Stewart, E.V. Bockstaele, M. De Loose, and B. V. Droogenbroeck, Food Chem. 2012, 134, 669-677

[15] B. Goueti, B. Kpadonou-Kpoviessi, L. Ahoussi, B. Glinma, A. Tchinda, L. Tchokouaha, J. Gbenou, B. Lenta, and S. Kpoviessi, J. Pharmacognos. Phytochem. 2023, 12 (3), 150–155

[16] K. P. F. O. Koné, Y. Soro, and S. Siaka, J. Soc. Ouest-Afr. Chim. 2017, 044, 15 - 22

[17] S. T. Bowen, H. W. Moise, G. Waring, and P. Man-Chiu, The J. Comp. Physiol. B, 1976, 55, 99–103

[18] M. A. Akanji, M. T. Yakubu, and M. I. Kazeem, J. Med. Sci. 2013, 13, 253–260

[19] M. A. Sonibare, T.O. Lawal, and O. O. Ayodeji, Inter. J. Pharmacol., 2011, 7 (4), 492–497

[20] P. Erastos, Z. Mbwambo, R. S. O. Nondo, N. Lall, A. Lubschagne, Spatula. 2011, 1 (2), 73–80

[21] G. A. Pesewu, R. R. Cutler, and D. P. Humber, J. Ethnopharmacol., 2008, 116 (1), 102–111.

[22] S. V. Fannang, V. Kuete, C. D. Mbazoa, J. I. Momo, H. T. Van-Dufat, F. Tillequin, E. Seguin, E. Chosson, J. Wandji, Chem. Nat. Compd., 2011, 59 (4), 404–407


  • There are currently no refbacks.

Creative Commons License
This work is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License.