Effect of Avocado Seed Ethanol Extract (Persea americana Mill) on Superoxide Dismutase (SOD1) and Histological Expression of Pancreas in Rats (Rattus norvegicus) with Diabetes Millitus
Fauziah Usman, Nurhaida Nurhaida, Binawati Ginting, Aulanni’am Aulanni’am
J. Pure App. Chem. Res. Vol 12, No 2 (2023), pp.
Submitted: February 28, 2023     Accepted: June 13, 2023     Published: August 28, 2023

Abstract



Diabetes mellitus causes damage to pancreatic β-cells due to increased oxidative stress, thereby reducing Superoxide Dismutase 1 (SOD1) activity. Activity of this enzyme can be increased by utilizing antioxidant compounds found in avocado seed extract. The aims of this study were to determine the increase in SOD1 expression and repair of pancreatic β-cells from the ethanol extract of avocado seeds (Persea americana Mill) as an alternative medicine for diabetes mellitus. The aim of this research to analyze the expression of pancreatic SOD1 and histopathology change of pancreas of DM rats. The expression of pancreatic SOD1 was measured by using immunohistochemical, and histopathology changes of pancreas were observed by using HE staining. This research used 20 Wistar rats aged of 2-3 moths, weighing of 150-200 grams. Diabetic rats induced by streptozotocin (STZ) with dose of 30 mg/kg BW intraperitoneally. Rats were divided into 5 groups, namely: group 1 (healthy rats), group 2 (diabetic rats), while the group of 3, 4 and 5 are therapeutic groups with dose of 300, 350 and 400 mg/kg BW of ethanol extract of avocado seeds for 14-day treatment. The results showed that the ethanolic extract of avocado seeds has a potential as an antidiabetic agents based on the increasing pancreatic SOD1 (p<0,05) significantly, increased SOD1 expression 7.6 ± 1.34, 11.6 ± 1.94 and 13.0 ± 2.82 in three different doses therapy (300, 350 and 400 mg/kg BW), and repairing structure of β-pancreatic cells on therapeutic groups.


Keywords : Diabetes mellitus, Avocado seed ethanol eextract, Superoxide dismutase, Hematoxylen-Eosin


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