Mass Spectrometry Analysis of Chromium-Binding Low-Molecular-Weight Serum Fractions

Authors

  • Anna Safitri University of Brawijaya
  • Aviva Levina School of Chemistry, the University of Sydney
  • Peter A Lay School of Chemistry, the University of Sydney

DOI:

https://doi.org/10.21776/ub.jpacr.2017.006.02.326

Keywords:

acetonitrile, Cr(III), low-molecular-weight, mass spectrometry, speciation

Abstract

The current work is focused on the characterization of the chromium(III)-containing low-molecular-weight (LMW) fractions of blood serum by mass spectrometry. The LMW fractions of serum binding-Cr(III) were collected by organic solvent precipitation using acetonitrile. The chromium(III) compounds used were [Cr(pic)3], where pic = 2-pyridinecarboxylato(-) or A, and the Cr(III) propionate complex, [Cr3O(OCOEt)6(OH2)3])+ or B. The new signals appeared in the mass spectra in the presence of added Cr(III) compounds, either A or B. This indicated that common LMW Cr containing serum fractions were formed on addition of Cr(III) to serum. The new signals contained three distinct peaks with a shift of 16, corresponding to ions of m/z +1083, +1099, and +1115 Da, which consisted of 4 Cr atoms with bridging ligands.

Downloads

Download data is not yet available.

Author Biography

  • Peter A Lay, School of Chemistry, the University of Sydney
    Director, Vibrational Spectroscopy Core Facility Research Portfolio Flagship Program Co-Leader, New Insights into disease and drug targets: from endogenous biological nanoscale vesicles to engineered nanoparticles Australian Institute of Nanoscale Science and Technology

References

[1].     Vincent, J. B.,  Proceedings Nut. Soc., 2004, 63, 41-47.

[2].     Pu, D., Vincent, J. B., Cassady, C. J., J. Mass Spectrom., 2008, 43, 773-781.

[3].     Vincent, J .B., J. Trace Elem. Med. Biol., 2014, 28, 397-405.

[4].     Vincent, J. B., Polyhedron, 2001, 20, 1-26.

[5].     Levina, A., Harris, H. H., Lay, P. A., J. Am. Chem. Soc., 2007, 129, 1065-1075 (Erratum p9832).

[6].     Vincent, J. B., Dalton Trans., 2010, 39, 3787-3794.

[7].     Feng, W., Li, B., Chai, Z., Zhang, P., Gao, Y., Zhao, J., Anal. Bioanal. Chem., 2003, 375, 363-368.

[8].     Crans, D. C., Woll, K. A., Prusinskas, K., Johnson, M. D., Norkus, E., Inorg. Chem, 2013, 52, 12262-12275.

[9].     Haraguchi, H., J. Anal. At. Spectrom., 2004, 19, 5-14.

[10].   Sanz-Medel, A., Montes-Bayón, M., Sánchez, M. L. F., Anal. Bioanal. Chem., 2003, 377, 236-247.

[11].   Sun, Y., Ramirez, J., Woski, S. A., Vincent, J. B., J. Biol. Inorg. Chem., 2000, 5, 129-136.

[12].   Speetjens, J. C., Parand, A., Crowder, M. W., Vincent, J. B., Woski, S. S.,  Polyhedron, 1999, 18, 2617-2624.

[13].   Nguyen, A., Mulyani, I., Levina, A., Lay, P. A., Inorg. Chem., 2008, 47, 4299-4309.

[14].   Becker, J.S., Mounicoua, S., Zoriy, M. P., Becker, J. S., Lobinski, R., Talanta, 2008, 76, 1183-1188.

[15].   Yates, J. R., Ruse, C. I., Nakorchevsky, A., Annu. Rev. Biomed. Eng., 2009, 11,  49-79.

[16].   Adkins, J. N., Varnum, S. M., Auberry, K. J., Moore, R. J., Angell, N. H., Smith, R. D., Springer, D. L., Pounds, J. G., Mol. Cell. Proteomics, 2002, 1, 947-955.

[17].   Tirumalai, R.S., Chan, K. C., Prieto, D. A., Issaq, H. J., Conrads, T. P., Veenstra, T. D., Mol. Cell. Proteomics, 2003, 2, 1096-1103.

 [18].  Chertov, O., Biragyn, A., Kwak, L. W., Simpson, J. T., Boronina, T., Hoang, V. M., Prieto, D. R. A., Conrads, T. P., Veenstra, T. D., Fisher, R. J., Proteomics, 2004, 4, 3243-3250.

[19].   Merrel, K.,Southwick, K., Graves, S. W., Esplin, M. S., Lewis, N. E., Thulin, C. D.,  J. Biomol. Tech., 2004 , 15, 238-248.

[20].   Nielsen, F. H., J. Trace Elem. Med. Biol., 2014, 31, 226-234.

[21].   Staniek, H., Krejpcio, Z., Iwanik, K., Food Chem. Toxicol. 2010, 48, 859-864

[22].   Stearns, D. M., Amstrong, W. H., Inorg. Chem., 1992, 31, 5178-5184.

[23].   Antsyshkina, A.S., Porai-Koshits, M. A., Arkhangel'skii, I. V., Diallo, I. N., Russ. J. Inorg. Chem., 1987, 32, 1700-1703.

[24].   Mulyani, I., In Vitro Biological Activities and Chemistry of Cr(III) Dietary Supplements, in Department of Chemistry, 2006, The University of Sydney: Sydney, Australia.

[25].   IsoPro 3.0, 1998, Senko, M.:Sunnyvale, CA.

[26].   Davis, C. M., Vincent, J. B., Arch. Biochem. Biophys., 1997, 339, 335-343.

[27].   Pivatto, M., Crotti, A. E. M., Lopes, N. P., Castro-Gamboa, I., Rezende, A., Viegas, Jr., C., Young, M. C. M., Furlan, M., Bolzani, V. S., J. Braz. Chem. Soc., 2005. 16, 1431-1438.

Downloads

Published

2017-05-04

Issue

Vol. 6 No. 2 (2017): Edition of May-August 2017

Section

Articles

License

Authors who publish with this journal agree to the following terms:

  1. Authors retain copyright and grant the journal right of first publication with the work simultaneously licensed under a Creative Commons Attribution-NonCommercial 4.0 International License that allows others to share the work with an acknowledgement of the work's authorship and initial publication in this journal.
  2. Authors are able to enter into separate, additional contractual arrangements for the non-exclusive distribution of the journal's published version of the work (e.g., post it to an institutional repository or publish it in a book), with an acknowledgement of its initial publication in this journal.
  3. Authors are permitted and encouraged to post their work online (e.g., in institutional repositories or on their website) prior to and during the submission process, as it can lead to productive exchanges, as well as earlier and greater citation of published work (See The Effect of Open Access).

How to Cite

Mass Spectrometry Analysis of Chromium-Binding Low-Molecular-Weight Serum Fractions. (2017). The Journal of Pure and Applied Chemistry Research, 6(2), 100-111. https://doi.org/10.21776/ub.jpacr.2017.006.02.326