Study of Ascorbic Acid as Iron(III) Reducing Agent for Spectrophotometric Iron Speciation
Antesar Elmagirbi, Hermin Sulistyarti, Atikah Atikah
J. Pure App. Chem. Res. Vol 1, No 1 (2012), pp. 11-17
Submitted: October 02, 2012     Accepted: October 02, 2012     Published: October 02, 2012
DOI: http://dx.doi.org/10.21776/ub.jpacr.2012.001.01.101

Abstract



The study of ascorbic acid as a reducing agent for iron(III) has been investigated in order to obtain an alternative carcinogenic reducing agent, hydroxylamine, used in spectrophotometric standard method based on the formation of a red-orange complex of Fe(II)-o-phenanthroline. The study was optimised with regards to ascorbic acid concentration as well as pH solution. The results showed that ascorbic acid showed maximum capacity as reducing agent of iron(III) under concentration of 4.46.10-4 M and pH solution of 1-4.Under these conditions, ascorbic acid reduced iron(III) proportionally and performed similarly to that of hydroxylamine.  The method gave result to linear calibration over the range of 0.2-2 mg/L withhigh accuracy of 97 % and relative standard deviation of less than 2 %. This method was successfully applied to assay iron speciation in water samples.

Keywords : Iron, speciation; ascorbic acid; o-phenanthroline; spectrophotometry
Full Text: PDF


References


Colter, A., and Mahler R.L. Drinking water, 2006, 589. [2] Safavi A.,Abdollahi,H. Microchem. J., 1999, 63, 211–217. [3] Aksu, Z., Calik, A., Dursun, A.Y., Demircan, Z. Process Biochem., 1999, 34,483–491. [4] Kari M.V. Ann. Zool. Fennici,., 1995, 32, 317-329. [5] Bakircioglu, Y., D. Bakircioglu, and N. Tokman. Anal. Chim. Acta., 2005, 547: 26–30. [6] Shuji K., and Hiroki H. Anal. Chim. Acta., 2003, 482,189–196. [7] Yang Z., Hou X. D., and Jones B. T. Microchem. J., 2002, 72: 49–54. [8] Sonia G.M., Mauro A. C., Pedro E., Felipe Y., Lourdes H. A., Giuseppe P., and Juan J. L. J. Chromatogr. A., 2005, 1064, 67–74. [9] Ortega C., and Cerutti S. J. Pharm. Biomed. Anal., 2004, 36: 721–727. [10] Qiu F. X., and Yao C. Fenxi Huaxue, 2000, 28,792. [11] Lu, M., Jia S. Y. and Zhang B. Yejin Fenxi, 2004, 24, 55–56. [12] Ma, W. X., and GeH. Y. Lihua, Jianyan Huaxue Fence., 2000, 36: 74–76. [13] Zhu H. Y., and Wang J. H. Lihua, Jianyan Huaxue Fence, 2000, 36, 391–393. [14] Nebahat D., and Fikriye T.E., Turk. J. Chem., 2003, 27, 315- 321. [15] Dong Y and Dayou Fu., Anal. Lett., 2011, 44 (1-3), 271-283. [16] American Public Health Association, APHA, Standard method for examination of water and wasterwater. 18th, 1992, American Public Health Association, Washington, 3-65-3-68. [17] Chris T,E ., Anita A, S., Rob A, B., Leo G, B., John M, N. Blood Cells Mol.Dis., 1998, 24 (3), 280-295.


Refbacks

  • There are currently no refbacks.