Formulation of M/A-Type Ointment Dosage From Ethanol Extract of White Plumeria Leaves (Plumeria alba l.) Against Candida albicans
Dian Riana Ningsih, Zusfahair Zusfahair, Dwi Kartika, Mei Lianasari
J. Pure App. Chem. Res. Vol 7, No 3 (2018), pp. 247-256
Submitted: May 09, 2018     Accepted: July 26, 2018     Published: September 02, 2018


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Candida albicans is one of fungi that can cause various infections. This fungal infection is known as candidiasis and some people often suffer from candidiasis. The sweaty and moisty skin is a factor that allows the growth of fungi causing skin diseases. The plants that can be used as antifungal is white plumeria leaves (Plumeria alba L.). Ethanol extract of white plumeria leaves contains alkaloid and saponin active compounds, to ease its use and to get the maximum effect, then white plumeria leaves are formulated into ointment dosage form. This research is to know the antifungal activity of extract, to formulate ointment and to know the characteristic of the extract. The research begins with determination of minimum inhibitory concentration (MIC) of extract against C. albicans, then formulation of ointment dosage. The physical properties of the ointment is tested including homogeneity test, pH, dispersive power, protection power, adhesion and hedonic and antifungal activity. The method used in determining MIC and the activity test of dosage is agar-well diffusion method. The MIC value of extract was 5 ppm with a 1.22 mm inhibition zone. Extract has a form of semisolid, white colour, distinctive odor, homogeneous, protective, pH of 5.07-5.59, dispersive power of 5,09-5,78 cm, adhesion of 1.00-2.33 seconds and antifungal activity of ointment at day 0 for concentrations of 5, 10 and 15 ppm respectively are 2.93; 5.2 and 7.87 mm and at day 15 for concentrations of 5, 10 and 15 ppm respectively are 3.68; 4.87 and 5.82 m

Keywords : antifungal; ethanol extract; Candida albicans; white plumeria; ointment
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[1] Kumamoto, C. A., Vinces, M. D. Annu. Rev. Microbiol. 2005, 59, 113–133. crossref

[2] Bae, G. Y., Lee, H. W., Chang, S. E., Moon, K. C., Lee, M. W., Choi, J. H., Koh, J. K. Int. J. Dermatol. 2005, 44 (7), 550–555. website

[3] Kobayashi, C. C. B. A., Fernandes, O. de F. L., Miranda, K. C., de Sousa, E. D., Silva, M. do R. R. Mycopathologia 2004, 158 (1), 49–52. website

[4] DiNubile, M. J., Hille, D., Sable, C. A., Kartsonis, N. A. J. Infect. 2005, 50 (5), 443–449. crossref

[5] Casari, E., Ferrario, A., Morenghi, E., Montanelli, A. New Microbiol. 2010, 33 (1), 69. website

[6] Brooks, G. F., Butel, J. S., Morse, S. A., Mudihardi, E. Jawetz, Melnick, & Adelberg’s mikrobiologi kedokteran, Salemba Medika, 2005.

[7] Ningsih, D. R., Zusfahair, Z., Purwati, P. Molekul 2014, 9 (2), 101–109. crossref

[8] Kumari, S., Mazumder, A., Bhattacharya, S. Int. J. PharmTech Res. 2012, 4 (1), 208–212. website

[9] Ansel, H. C. UI Press 2005.

[10] Ningsih, D. R., Zusfahair, Kartika, D., Lestari, I. T. In IOP Conference Series: Materials Science and Engineering, 2017, Vol. 172, pp. 1-9. website

[11] Bayuaji, T. S., Astuti, I. Y., Dhiani, B. A. Pharmacy 2012, 9 (3), 57-64. website

[12] Wasitaatmadja, S. M. Univ. Indones. Jakarta 1997.

[13] Kumaresan, A., Deepthi, G., Anandh, V., Prathap, S. Int. J. Pharm. Sci. Heal. care 2012, 1 (2), 71–81. website

[14] Lieberman, H. A., Rieger, M. M., Banker, G. S. Pharmaceutical Dosage Forms--Disperse Systems, M. Dekker, 1998, Vol. 2.

[15] Faatih, M. J. Penelit. Sains Teknol. 2005, 6 (1), 35–48. website

[16] Berghe, D. A. Vanden, Vlietinck, A. J. Methods Plant Biochem. Assay Bioactivity. Acad. Press. London 1991, 6, 47.

[17] McCarthy, P. J., Pitts, T. P., Gunawardana, G. P., Kelly-Borges, M., Pomponi, S. A. J. Nat. Prod. 1992, 55 (11), 1664–1668. website

[18] Mycek, M. J., Harvey, R. A., Champe, P. C., Fisher, B. D. Edisi 2001, 2, 101–103.

[19] Naibaho, O. H., Yamlean, P. V. Y., Wiyono, W. Pharmacon 2013, 2 (2), 27–33. website

[20] Ulaen, S. P. J., Banne, Y., Suatan, R. A. J. Ilm. Farm. Poltekkes Manad. 2012, 3 (2), 45-49. website

[21] Susilowati, E. P. IJMS-Indonesian J. Med. Sci. 2013, 1 (2), 29-34. website

[22] Wibowo, S. A., Budiman, A., Hartanti, D. JRST J. Ris. SAINS DAN Teknol. 2017, 1 (1), 15–21. website

[23] Zulfa, E., Prasetyo, T. B., Murrukmihadi, M. J. Pharmascience 2017, 4 (1), 18-24. website


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