A Validated Ultra Performance Liquid Chromatography Method for Quantification of Metformin in Human Plasma
Vycke Yunivita Kusumah Dewi, Atu Purnama Dewi, Budi Setiabudiawan, Edhyana Sahiratmadja, Triana Nurul Meirina, Harold Eka Atmaja, Rovina Ruslami
J. Pure App. Chem. Res. Vol 7, No 3 (2018), pp.
Submitted: April 24, 2018     Accepted: November 05, 2018     Published: November 05, 2018

Abstract



Metformin is oral hypoglycemic or blood sugar-lowering drug which is the first-line drug in the treatment of diabetes mellitus type 2. This study presents the validated of UPLC-UV method for the determination of metformin in human plasma.

Metformin levels were measured using UPLC with UV detector and liquid-liquid extraction method. Separation was carried out on a Acquity UPLC HSS T3 100 mm × 2.1 mm i.d. column (1.8 μm partikel size) using gradient elution of acetonitrile and phosphate buffer 0.02 M (0.6 ml/min) as mobile phase at 30°C. The analyt was monitored at 236 nm. No endogenous substances were found to interfere with the peaks of drug and internal standard.

The value of percent deviation and the coefficient variation obtained respectively less than the percentage set in the FDA guidelines. The linearity factor values were more than 0.997 and LOD is 0.01 µg/ml. UPLC with UV detector is able to analyze metformin in a short time with good precision and accuracy which is usefull for bioequivalency/bioavailability studies.


Keywords : Validated analysis, Metformin plasma, UPLC


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