The Toxicity Effect of Organophosphate (Diazinon) towards Duodenum Histopathology and The Activity of Superoxide Dismutase (SOD) Serum in Rats (Rattus Norvegicus)
S. Efi Ulansari, Anna Roosdiana, Dyah Ayu Oktavianie A. P.
J. Pure App. Chem. Res. Vol 7, No 2 (2018), pp. 109-115
Submitted: October 23, 2017     Accepted: February 13, 2018     Published: May 06, 2018


Cover Image

Diazinon is an organophosphate insecticide that can inhibit acetyl-cholinesterase competitively and increase the production of Reactive Oxygen Species (ROS). ROS affect the activity of antioxidant enzymes such as superoxide Dismutase (SOD), and simultaneously ROS cause damage to the cells and duodenum tissue. The toxicity effect of diazinon was investigated by measuring the activity of SOD serum and histopathology of the duodenum in rats (Rattus norvegicus). The activities of SOD serum were measured by using spectrophotometry, and histopathology changes of the duodenum were observed by using HE staining. Rats were divided into 4 groups, negative control group (K-), P1, P2, and P3 groups which were administrated with diazinon for 8 weeks orally with a dose of 20 mg/kgBW, 40 mg/kgBW, and 60 mg/kgBW respectively. The results showed that administration of diazinon orally decreased SOD activity significantly and caused damage of duodenal villi such as hyperplasia of epithelial cells, epithelial erosion, fatty degeneration, hyperplasia of Liberkuhn gland cells, and hemorrhage in the lamina propria.

Full Text: PDF


[1] Mishra, Badade Z.G., Rastogi S.K, and Singh S, IOSR Journal of Dental and Medical Sciences (IOSR-JDMS), 2013, 7(6), 20-24.

[2] Zheng Q, Oliver K, Won YK, Pope C.N, Toxicol. Sci., 2000, 55, 124-132

[3] Indraningsih and Yulvian Sani, WARTAZOA, 2004, 14(1), 1-13.

[4] Eleršek, Tina and Metka Filipic, InTech Europe. Croatia, 2011, 243-260.

[5] Debski, Bogdan, Bogdan Feliks Kania, and Tomasz Kuryl, Ekológia (Bratislava), 2007, 26(1), 68–82.

[6] Abou-Donia MB, Arch. Env. Health. 2003, 58(8), 484-497.

[7] Uchendu, Chidiebere, Suleiman F. Ambali and Joseph O. Ayo, Afr. J. Agri. Res., 2012, 7(18), 2720-2728.

[9] Cox, Caroline, Journal of Pesticide Reform, 2000, 20(2), 15-21.

[8] El-Shenawy, N. S., R. A. Al-Eisa, F. El-Salmy, and O. Salah, Curr. Zool., 2009, 55(3), 219-226.

[10] Baconi, Daniela Luiza, Maria Bârca, Gina Manda, Anne-Marie Ciobanu, and C. Balalau, Rom. J. Morphol. Embryol., 2013, 54(2), 349-356.

[11] Gupta, Ramesh C, Handbook of Toxicology of Chemical Warfare Agents, 2009, Elsevier Academic Press. United States of America. pp. 32-33.

[12] Georgievskii, V. I., B. N. Annenkov, and V. T. Samokhin, Material Nutrition of Animals: Studies in the Agricultural and Food Sciences, 2013, Butterworths & Co. (Publishers) Ltd. London. pp. 160-161.

[13] Mescher, Anthony L., Junqueira's Basic Histology, Text & Atlas 13th Edition, 2013, McGraw-Hill Education.United States of America. pp. 309-315.

[14] Caramori, G. and A. Papi, Int J Pharm Bio Sci, 2004, 59(2), 170-173.

[15] Sharma, A, S. Bansal, and R.K. Nagpal, Indian J. Pediatr., 2003, 70(9), 715-717.

[16] Rady, Mohamed Ibrahim, Braz. Arch. Biol. Technol., 2009, 52(2), 317-326.

[17] Kaur, Gurpreet, A. K. Jain and Sandeep Singh, J. Genet., 2016, 1-15.

[18] Gina, Luh Putu, and Chanif Mahdi. J. Pure App. Chem. Res., 2014, 3 (3), 131-137.

[19] Cemelli, E., A. Baumgatner, and D. Anderson, Mutat. Res., 2009, 681, 51-67.

[20] Fujii, J., Y. Iuchi, S. Matsuki, and T. Ishii, Asian J. Androl, 2003, 5, 231-242.

[21] Lee, J., N. Koo, and D.B. Min, Compr. Rev. Food Sci. Food Saf., 2004, 3, 21-33.


  • There are currently no refbacks.

Creative Commons License
This work is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License.