Chemical Composition of Oil Fraction Kaffir Lime (Citrus hystrix DC) as Antibacterial Activity of E.coli
Rahmatika Ayu Habsari, Warsito Warsito, Noorhamdani Noorhamdani
J. Pure App. Chem. Res. Vol 7, No 1 (2018), pp.
Submitted: August 07, 2017     Accepted: November 29, 2017     Published: January 04, 2018


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The purpose of this research is to know the composition of fraction oil kaffir lime which is consist as antibacterial activity E.coli. This research uses kaffir lime oil from a branch for fractional distillation use PiloDist 104-VTU (number the stages 120), and reflux ratio 20/10 with 5 mbar pressure. Oil kaffir lime composition analysis use GC-MS (type Shimadzu QP 2010S) with a gas helium on speed 3mL/minute. Antibacterial activity assay use agar well diffusion assay which does in three concentration they are 500 µL/mL, 300 µL/mL, 100 µL/mL. The result of oil fraction kaffir lime has five fraction oil based on boiling point interval, they are A fraction oil (63,00 – 70,010 C), B fraction (71,30 – 70,800 C),  C fraction (74,50 – 74,200 C),  D fraction (74,20 – 74,000 C) and E fraction (72,90 – 91,100 C). The result of this research obtained five oil fraction they are monoterpene oxygenated (MO) except A oil fraction with monoterpene hydrocarbon composition (MH) 12,1%. The main component of a fraction which MO compound they are citronella, linalool and isopulegol, while in MH compound they are sabine, β-pinen, β-micren and limonene. The result of antibacterial activity assay obtained on highest concentration it is 500 µL/mL. Antibacterial activity assay also depends on fraction composition with higher composition of MO. The highest MO components of oil fraction on C oil fraction which has MO component such as citronella 74,94%; linalool 20,13%; and isopulegol 3,08%.

Keywords : Kaffir lime oil fraction, antibacterial activity, E.coli, monoterpenoid hydrocarbon, monoterpenoid oxygenated
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[1]. Nascimento, G. G. F., Juliana .L, Paulo C. F, Giuliana L. S, Braz J Microbiol, 2000, 31, 247-256.

[2]. Souza, C. A. S., Avancini, C. A. M., Wiest, J. M., Braz. J. Vet. Res. Anim. Sci., 2000, 37(6), 429-433.

[3]. J. Wungsintaweekul, W. Sitthithaworn, W. Putalun, H. W. Pfeifhoffer and A. Brantner, Songklanakarin J. Sci. Technol., 2010, 32(6), 589-598.

[4]. Srisukh, V., N. Bunyapraphatsara, A. Pongpan, W. Tungrugsasut, S. Puttipipatkhachorn, W. Oniam, T. Karawamitr, S. Bunsiriluk, and W. Thongbainoi, MUJPS, 2012, 39 (2), 15-27.

[5]. Warsito, Noorhamdani A. S, Sukardi, Suratmo, Chemical Compositions and Antioxidant Activity of Fraction and Kaffir Lime (Citrus Hystrix) Oils, 2013, Superior Report of Universitas Brawijaya.

[6]. Adams R. P., Identification of Essential Oils components by Gas Chromatography/Quadrupole Mass Spectroscopy, 4th ed, 2001, Allured Publishing Corp, Carol Stream, IL, USA.

[7]. Sarikurkcu, C., G. Zengin, M. Oskay, S. Uysal, R. Ceylan, A. Aktumsek, Ind Crops Prod., 2015, 70, 178-184.

[8]. Loh, F. S, Rita Muhamad Awang, Dzolkhifli Omar and Mawardi Rahmani, J. Med. Plants Res., 2011, 5(16), 3739-3744.

[9]. Parekh J., Chanda S., Turkish J. Biol., 2007, 31, 53-58.

[10]. Chanthaphon, S., Suphitchaya .C, and Tipparat .H, Songklanakarin J. Sci.

Technol., 2008, 30 (1), 125-131.

[11]. Hussain, A. I., Anwar, F., Hussain Sherazi, S. T., and Przybylski, R, Food Chem, 2008, 108(3), 986-995.

[12]. Bakkali, F., Averbeck, S., Averbeck, D., Idaomar, M., Food Chem Toxicol, 2008, 46(2), 46, 446-475.

[13]. Sokovic M. and Griensven L. J. L. D, Eur J Plant Pathol., 2006, 116-211.

[14]. Magiatis P., Melliou E., Skaltsounis A.L., Chinou I. and Mitaku S, Planta medica., 1999, 65, 749.

[15]. Wu N., Fu Y. J., Kong Y., Zu Y. G. and Liu X. L., Environ Toxicol Pharmacol., 2010, 32(1), 63-8.

[16]. Zhu, L, Y. J Tian, Y. C Yin and S. M. Zhu, IJFS, 2012, 24, 19-25.

[17]. Kurita, N., Miyaji, M., Kurane, R., Takahara, Y. and Ichimura, K, Agric Biol Chem., 1981, 45, 945–952.

[18]. Pelczar, M. J., Chan, E. C. S. and Krieg, N.R. Control of microorganisms, the control of microorganisms by physical agents, 2th Edition, 1988, New York, McGraw-Hill International..

[19]. Sivarooban, T., Hettiarachchy, N. S., Johnson, M. G., J Food Prot., 2008, 71, 2105–2109.

[20]. Kurita, N., Miyaji, M., Kurane, R., Takahara, Y. and Ichimura, K., Agric Biol Chem., 1979, 43, 2365–2371.

[21]. Dormans, H. J. D., Deans, S. G, J Appl Microbiol, 2000, 88, 308–316.

[22]. Gill, A. O., Holley, R. A., Appl Environ Microbiol, 2004, 70, 5750–5755.

[23]. Moosavy, M. H., Basti, A. A., Misaghi, A., Zahraei, T., Abbasifar, R., Ebrahimzadeh, H. A., Alipour, M., Emami Razavi, N., Gandomi, H., Noori, N., Food Res Int., 2008, 41, 1050–1057.

[24]. Chan, A. C., Ager, D., Thompson, I. P., J Microbiol Methods, 2013, 93, 209–217.

[25]. Andrews R. E., Parks L. W. and Spence K. D, Appl Environ Microbiol, 1980, 40, 301.

[26]. Uribe S., Ramirez J. and Pena A, Am Soc Microbiol, 1985, 161, 1195

[27]. Donsi F., Annunziata M., Sessa M. and Ferrari G, Lebensm. Wiss. Technol., 2011, 44, 1908-1914

[28]. Delaquis P. J., Stanich K., Girard B., Mazza G, Int J Food Microbiol, 2002, 74,101–109.

[29]. Burt, S., Int J Food Microbiol, 2004, 94, 223-253.

[30]. Edris, A. E., Phytother Res, 2007, 21, 308–323.

[31]. Dormans, H. J. D., Deans, S. G, Phytother Res, 2000, 88, 308–316.

[32]. Lambert, R. J. W., Skandamis, P. N., Coote, P. J., Nychas, G. J. E., J Appl Microbiol, 2001, 91, 453–462.


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