Mass Spectrometry Analysis of Chromium-Binding Low-Molecular-Weight Serum Fractions
Anna Safitri, Aviva Levina, Peter A Lay
J. Pure App. Chem. Res. Vol 6, No 2 (2017), pp. 100-111
Submitted: February 03, 2017     Accepted: March 21, 2017     Published: May 04, 2017
DOI: http://dx.doi.org/10.21776/ub.jpacr.2017.006.02.326

Abstract


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The current work is focused on the characterization of the chromium(III)-containing low-molecular-weight (LMW) fractions of blood serum by mass spectrometry. The LMW fractions of serum binding-Cr(III) were collected by organic solvent precipitation using acetonitrile. The chromium(III) compounds used were [Cr(pic)3], where pic = 2-pyridinecarboxylato(-) or A, and the Cr(III) propionate complex, [Cr3O(OCOEt)6(OH2)3])+ or B. The new signals appeared in the mass spectra in the presence of added Cr(III) compounds, either A or B. This indicated that common LMW Cr containing serum fractions were formed on addition of Cr(III) to serum. The new signals contained three distinct peaks with a shift of 16, corresponding to ions of m/z +1083, +1099, and +1115 Da, which consisted of 4 Cr atoms with bridging ligands.


Keywords : acetonitrile, Cr(III), low-molecular-weight, mass spectrometry, speciation
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