A Study on Brown Seaweed Therapy ( Sargassum sp.) toward MDA Levels and Histological Improvement on Rat Foot Suffering Rheumatoid Arthritis
Fauziah Fauziah, Aulanni'am Aulanni'am, Chanif Mahdi
J. Pure App. Chem. Res. Vol 2, No 3 (2013), pp. 102-107
Submitted: July 11, 2013     Accepted: September 07, 2013     Published: September 12, 2013
DOI: http://dx.doi.org/10.21776/ub.jpacr.2013.002.03.147

Abstract



Rheumatoid arthritis (AR), an autoimun disease, is characterized by the inflammation in the joint area caused an excessive of free radicals. An excessive of free radicals in the body cause oxidative stress, that increasing levels of malondialdehyde (MDA) as an indicator of lipid peroxidation and the decreasing levels of anti-oxidants. The treated with extract of brown seaweed (Sargassum sp.) intended to find out the MDA levels in serum and the histological of the foot joints rheumatoid arthritis rats. Malondialdehyde levels are determined through a TBA test (Thio Barbituric acid), meanwhile the histological of the rat foot joints was determined by Hematoxylen-Eosin staining (HE). The results showed the brown seaweed extract therapy (Sargassum sp.) was significantly (p <0.01) reduce levels of malondialdehyde (MDA) in the serum of 21,24% and improving histological foot joints rheumatoid arthritis rats.


Keywords : Rheumatoid arthritis; Malondialdehyde; Herbal therapy; Brown seaweed
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References


Kramer, I., A. Wibulswas,. D. Croft,. and E. Genot,. 2003. Rheumatoid Arthritis: Targeting The Poliferation Fibroblast. in Meijer, L. Jézéquel, A. and Roberge, M. Progress in Cell Cycle Reaserch. Vol 5:59-70. [2]Direktorat Bina Farmasi Komunitas dan Klinik Dirjen Bina Kefarmasiandan alat Kesehatan Departemen Kesehatan. 2006. Pharmaceutical Care untuk Pasien Artritis Rematoid. Jakarta [3]Lee DM., ME. Weinblatt, 2001. Rheumatoid Arthritis Lancet; 358:903-911 [4]Algameta, ED. 2009, Uji aktivitas antioksidan tablet effervescent dewanduri (Eugenia uniflora L. ) dan sambilato ( Andrographis paniculata) pada tikus yang dibebani glukosa. Skripsi thesis, universitas Muhammadiyah surakarta. [5]Lim, S.N., P.C.K. Cheung, V.E.C. Ooi and P.O. Ang. 2002. Evaluation of Antioxidative of Extracts from a Brown Seaweed, Sargassum siliquastrum. J. Agric. Food Chem. 50: 3862-3866. [6]Botutihe, D.N. 2010. Effects of Extracts of the Brown Seaweed (Sargassum duplicatum Bory) against of Free radical's profile and protein kinase C pulmonary rats (Rattus norvegicus) is being exposed Benzo[A]Piren. Tesis (In Indonesia). Universitas Brawijaya. Malang. Hal 49. [7]O’Dell JR. Anticytokine therapy- a new era in the treatment of rheumatoid arthritis. New Engl J. Med. 1999; 340: 310-312 [8]Deaney CL., Feeyik., CM. Forrest, 2001. Level of lipid peroxidation products in acronic inflammatory disorder. Res Comm. Mol J. Patol Prharmacol.; 110: 87-95 [9]Bruch, C.G. and D.P Janet,. 2002. Oxidative Stress in Critically Ill Patients. AACN's bimonthly scientific journal. 11:543-551. [10]Nicolas Fabre and Isabelle Rustan, Edmond de Hoffmann dan Joe¨lle Quetin-Leclercq. 2001. Determination of Flavone, Flavonol, and Flavanone Aglycones by Negative Ion Liquid Chromatography Electrospray Ion Trap Mass Spectrometry American Society for Mass Spectrometry. Published by Elsevier Science Inc. [11]Burcham PC, 1998. Genotoxic lipid peroxidation products : their DNA damaging properties and role in formation of endogenous DNA adducts. Mutagenesis, 13 : 287 – 305.


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